Dulbecco's Modified Eagle's Medium (DMEM) (1X), Liquid
(High Glucose)
With
4500 mg/L D-glucose
Without
L-glutamine
Catalog
Number LM 001-01
Storage
Temperature 2~8°C
Product
Description
For animal
cell culture, Eagle’s Medium have been developed and modified since the
original formulation appeared in the literature. Dulbecco’s Modified Eagle’s Medium
(DMEM) is a modification of Minimum Essential Medium (MEM) that contains a
higher concentration of amino acids and vitamins, as well as additional supplementary
components, such as D-glucose (low or high, 1000 mg/L or 4500 mg/L,
respectively).
LM 001-01 contains
the high D-glucose concentration of 4500 mg/L, but no L-Glutamine. Add
aseptically 20 mL of sterile 200 mM L-glutamine solution (LS 002-01) per
1 L of medium before use. The selection of a nutrient medium is strongly
influenced by (1) type of cell, (2) type of culture (monolayer, suspension,
clonal), and (3) degree of chemical definition necessary. It is important to
review the literature for recommendations concerning medium, supplementation
and physiological parameters required for a specific cell line.
Storage/Stability
The liquid
medium should be stored at 2~8°C in the
dark. Deterioration of the liquid medium may be recognized by (1) precipitate or
particulate matter throughout the solution, (2) cloudy appearance, (3) color
change, and/or (4) pH change. The nature of supplements added may affect
storage conditions and shelf life of the medium. Product label bears expiration
date.
Biological
Performance Characteristics
The
growth-promoting capacities of the liquid media are tested in a medium
containing 10% FBS using an appropriate cell line(s). Growth rates are examined
through three subculture generations and compared with parallel cultures grown in
standardized control medium. Cells are counted and growth is plotted as a
logarithmic function of time in culture, and seeding efficiency, doubling time,
and final cell densities are determined. During the testing period cultures are
examined microscopically for a typical morphology and evidence of cytotoxicity.
Precautions
