CMRL 1066 Medium (1X), Liquid
With L-glutamine
With sodium bicarbonate

Catalog Number LM 013-01
Storage Temperature 2~8°C

Product Description
CMRL 1066 Medium, a simpler version of Medium 199, was developed in the late 1950’s by Connaught Medical Research Laboratories. In the early days, CMRL 1066 was used for in vitro cultivation of mouse L-strain cells in serum-free medium. But this medium with serum can be used for supporting growth of various cells.

LM 013-01 contains 100 mg/L L-glutamine and 2200 mg/L sodium bicarbonate. The selection of a nutrient medium is strongly influenced by (1) type of cell, (2) type of culture (monolayer, suspension, or clonal), and (3) degree of chemical definition necessary. It is important to review the literature for recommendations concerning medium, supplementation and physiological parameters required for a specific cell line.

Storage/Stability
Liquid CMRL 1066 Medium should be stored at 2~8°C in the dark. Deterioration of the liquid medium may be recognized by (1) precipitate or particulate matter throughout the solution, (2) cloudy appearance, (3) color change, and/or (4) pH change. The nature of supplements added may affect storage conditions and shelf life of the medium. Product label bears expiration date.

Biological Performance Characteristics
The growth-promoting capacities of CMRL 1066 Medium are tested in a medium using an appropriate cell line. Growth rates are examined through three subculture generations and compared with parallel cultures grown in standardized control medium. Cells are counted and growth is plotted as a logarithmic function of time in culture, and seeding efficiency, doubling time, and the final cell density are determined. During the testing period cultures are examined microscopically for a typical morphology and evidence of cytotoxicity.

Precautions
For In Vitro Use Only