Williams’ Medium E, Liquid
With L-glutamine
With Sodium bicarbonate
With Phenol red

Catalog Number LM 017-02
Storage Temperature 2~8°C

Product Description
Williams' Medium E was developed by Williams team for the isolation and maintenance of adult rat liver epithelial cells in 1971. The medium was developed during explore the culturing adult liver cells. So, the medium was supported to long-term culture of adult liver epithelial cells.

LM 017-02 is Williams' Medium with sodium bicarbonate, L-glutamine and phenol red.

Storage/Stability
The liquid medium should be stored at 2~8°C in the dark. Deterioration of the liquid medium may be recognized by (1) precipitate or particulate matter throughout the solution, (2) cloudy appearance, (3) color change, and/or (4) pH change. The nature of supplements added may affect storage conditions and shelf life of the medium. Product label bears expiration date.

Biological Performance Characteristics
The growth-promoting capacities of Williams’ Medium E are tested in a medium containing 5~10% FBS using an appropriate cell line(s). Growth rates are examined through three subculture generations and compared with parallel cultures grown in standardized control medium. Cells are counted and growth is plotted as a logarithmic function of time in culture, and seeding efficiency, doubling time, and the final cell density are determined. During the testing period cultures are examined microscopically for a typical morphology and evidence of cytotoxicity.

Precautions
For In Vitro Use Only