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H > Product > Molecular Biology Products > Microbial Liquid Media > Microbial Liquid Media > SOC Medium
SOC Medium, liquid
SOC Medium, liquid
Cat. No. MM 006-01
Size 1㎖×50, 100㎖, 500㎖
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제품설명
SOC mediumE. coli를 plasmid DNA 등의 외래 DNA로 형질전환시킬 때 널리 사용되는 배지이다.

CaCl2와 heat-shock을 사용하여 세포에 외래 DNA를 삽입하고 SOC 액상 배지를 4배 부피 정도 첨가한 후 45 분 동안 배양하여 SOB agar plate*에 도말한다.

*SOB agar plate 제조방법

900㎖의 Cell/Tissue Culture Grade Water (LS 016-01)에 20g의 tryptone, 5g의 yeast extract, 그리고 0.5g의 NaCl을 첨가하고 완전히 녹인다. 10 ml의 250 mM KCl (1.86g을 100 ml의 Water에 녹여둔다.)을 첨가한다.

pH 7.0을 맞춘다. 15g의 agar를 첨가한다. 최종 부피 1ℓ를 맞춘다. Autoclaving (121℃, 15 lb/sq. in., 20 min)하여 멸균한다.

65℃로 식혀 5㎖의 멸균된 2 M MgCl2 (19g을 100 ml의 Water에 녹여 autoclaving해둔다.)과 적절한 항생제(selection agent, e.g. ampicillin)를 첨가한다. Petri-dish에 적당량을 부어 상온에서 고형화한다.

Product Name Description Cat.No. Recommended

Storage Temp.
Size
SOC Medium,

liquid
Components

20.0 g/L Bacto-Tryptone

5.0 g/L Bacto-Yeast Extract

0.5 g/L NaCl

2.5 mM KCl

10.0 mM MgCl220.0 mM Glucose

pH 7.0

Autoclaved (121°C, 15 lb/sq. in., 20 min; Tryptone,

Yeast Extract, NaCl, KCl, MgCl2)

Sterile-filtered (Glucose)

Bacterial culture tested

Transformation tested
MM 006-01 Store at 2-8℃ 1㎖×50

100㎖

500㎖
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