Continuous Cell Line and Related JBI Products

	다음 표는 많이 이용되고 있는 있는 세포주 중 일부의 특징과 JBI에서 제공하고 있는 배양 배지 및 혈청을 나타낸 것이다.
	Cell Line Origin Comments Recommended Medium Related JBIProducts(Cat. No.)   293  Human Permanent line of primary human embryonal kidney transformed by sheared human adenovirus type 5 (Ad5) DNA. The cells express the transforming gene of adenovirus 5. The line is excellent for titrating human adenoviruses  MEM + NEAA  + 1 mM Na·Pyruvate  + 10% heat-inactivated HS or MEM + NEAA  + 1 mM Na·Pyruvate  + 10% heat-inactivated FBS LM 007-01 LM 007-07 LS 005-01 LS 013-01 S 001-01 S 104-01   3T3 (A31)  Mouse Balb/c fibroblast. Similar preparation, growth and properties to Swiss 3T3, but from the inbred mouse strain Balb/c.  DMEM + 10% FBS LM 001-07 S 001-01 S 001-03 S 001-06   3T3 (Swiss albino)  Monkey Fibroblast cell originally prepared from disaggregated random bred Swiss mouse embryos. Adherent, highly contact inhibited, widely used for oncogene research.  DMEM + 10% FBS LM 011-01 S 001-01 S 001-03 S 001-06   B95-8 (A31)  Mouse Marmoset suspension lymphoblastoid cells, leak infections EBV after stressing. Popular choice as a convenient, easy to grow producer of infectious virus for EBF immortalization.  RPMI 1640 + 10% FBS LM 001-07 S 001-01 S 001-03 S 001-06   BeWo  Human Choriocarcinoma. The first human endocrine cell to be maintained in continuous culture. Epithelial-like, placental origin and secretes a spectrum of placental hormones. The cells are positive for keratin by immunoperoxidase staining.  Ham's F-12K + 15% FBS LM 010-53 S 001-01 S 001-03 S 001-06   BHK-21 (C-13)  Hamster Male baby Syrian hamster kidney cells clone C-13, adherent. This line has been used as a host for transformation with expression vectors containing selectable and amplifiable marker DNAs.  MEM + NEAA  + 1 mM Na·Pyruvate  + 10% FBS LM 007-01 LM 007-07 LS 005-01 LS 013-01 S 001-01 S 001-03 S 001-06   BS-C-1  Monkey African green monkey kidney. Epithelial-like, adherent cells that show characteristic morphological changes after infection with SV 40 virus. Also popular for transfection studies, especially with SV 40-based vectors.  MEM + NEAA + 10% FBS LM 007-01 LS 005-01 S 001-01 S 001-03 S 001-06   C3H/10T1/2  Mouse Contact-inhibited embryo fibroblast from C3H mice. Nontumorigenic, do not spontaneously transform, no endogenous leukemia or sarcoma virus detectable; highly susceptible to transformation by chemical agents. Differentiate into myoblasts, used to clone the myo D gene.  MEM + 10% FBS  or  BME  + 10% heat-inactivated FBS LM 007-01 LM 012-01 S 001-01 S 001-03 S 001-06 S 101-01   C6  Rat Chemically induced rat glial tumor cell. Fibroblastic. Secretes S-100 protein.  Ham's F-10 + 15% HS  + 2.5% FBS LM 009-01 S 004-01 S 001-01 S 001-03 S 001-06   CCRF-CEM  Human Children's Cancer Research Foundation. T-lymphoblastoid suspension cells from a 4-year-old female with acute lymphoblastic leukemia.  RPMI 1640  + 4.5 g/L glucose  +10 mM HEPES  + 1 mM Na·Pyruvate  + 10% FBS LM 011-01 LM 011-51 LS 001-02 BB 001-01 LS 013-01 S 001-01S 001-03 S 001-06   CHO-K1  Hamster Adherent epithelial-like cells isolated from Chinese hamster ovaries, widely used for mutation studies. The cells require proline in the medium for growth  Ham's F-12K + 1.5 g/L NaHCO3 + 10% FBS LM 010-52 S 001-01 S 001-03 S 001-06   CHO/dhFr-  Hamster Adherent epithelial-like cells isolated from Chinese hamster ovaries. The cells are deficient in dihydrofolate reductase  IMDM  + 0.1 mM hypoxanthine  + 0.016 mM thymidine  + 10% FBS LM 004-01 LM 002-04 S 001-01 S 001-03 S 001-06
	Continuous Cell Line and Related JBI Products (CONTINUED)
	Cell Line Origin Comments Recommended Medium Related JBIProducts(Cat. No.)   Clone M-3  Mouse Melanin-producing cell line. Adapted to culture rom a melanoma in C x DBS F1 hybrid mice. Forms melanotic tumors in mice.  Ham's F-10 + 15% HS + 2.5% FBS LM 009-01 S 001-01 S 001-03 S 001-06 S 004-01   COS-1  Monkey Derivate of CV-1. Transformed by origin of replication defective mutant of SV40 which codes for wild type T antigen. The cells contain a single integrated copy of the complete early region of the SV40 genome.  DMEM + 10% FBS LM 001-07 S 001-01 S 001-03 S 001-06   COS-7  Monkey Derivate of CV-1. This line was transformed with an origin defective mutant of SV40 which codes for wild-type T antigen.  DMEM + 10% FBS LM 001-07 S 001-01 S 001-03 S 001-06   CRFK  Cat Cat kidney epithelial cells, used extensively for cat viral studies.  MEM + NEAA + 1 mM Na·Pyruvate + 10% HS LM 007-01 LM 007-07 LS 005-01 LS 013-01 S 001-01 S 001-03 S 001-06   CV-1  Human African green monkey kidney. Adherent, fibroblastic cells. Suitable host for SV 40 infection. Show characteristic morphological changes and are popular as transfectable cells, especially for SV40-based vectors  MEM + NEAA + 1 mM Na·Pyruvate + 10% FBS LM 010-53 S 001-01 S 001-03 S 001-06   Daudi  Human Burkitt lymphoma, B-lymphoblastoid EBV-positive suspension cell. Extensively used in the study of oncogenesis and interferon research.  RPMI 1640 + 4.5 g/L glucose +10 mM HEPES + 1 mM Na·Pyruvate + 10% FBS LM 011-01 LM 011-51 LS 001-02 BB 001-01 LS 013-01 S 001-01 S 001-03 S 001-06   Detroit 562  Human Derived from metastatic cells from a carcinoma of the pharynx. Adherent epithelial-like cells.  MEM + NEAA + 1 mM Na·Pyruvate + 0.1% lactalbumin hydrolysate +10% FBS LM 007-01 LM 007-07 LS 005-01 LS 013-01 S 001-01 S 001-03 S 001-06   Don  Hamster Pseudodiploid Chinese hamster lung fibroblast-like cell.  MEM + 10% FBS or McCoy's 5A + 10% FBS LM 007-01 LM 005-01 S 001-01 S 001-03 S 001-06   EB-3  Human Burkitt lymphoma, B-lymphoblastoid EBV-positive suspension cell.  RPMI 1640 + 10% FBS LM 011-01 S 001-01 S 001-03 S 001-06   ES-D3  Mouse Pluripotent embryo stem-cell line isolated from the blastocyst of 129/Sv + C/ + P mouse. Spontaneously differentiate into embryonic structures, can be maintained as undifferentiated cells by growth on feeder layers or medium containing LIF-treated cells. Can be transfected and knockouts can be achieved by homologous recombination (gene targeting). Embryonic stem cells injected into blastocysts can colonize the germ line for transgenic mouse production.  DMEM + 15%  FBS+ 100 (M Mercap. LM 001-07 S 001-01 S 001-03 S 001-06   FDCP-1  Mouse Factor-dependent continuous cell lines. Paterson Laboratories. Diploid myeloid cells isolated from bone marrow culture of DBA/2 mice. IL-3 dependent for growth, nonmalignant in syngeneic mice, capable of differentiation into neutrophils and macrophage-like cells.  RPMI 1640 + 10% FBS LM 011-01 S 001-01 S 001-03 S 001-06   GH3  Rat Wister rat pituitary tumor epithelial cell line. Secretes growth hormone and prolactin, both regulated by hydrocortisone.  Ham's F-10 + 15% HS + 2.5% FBS LM 009-01 S 004-01 S 001-01 S 001-03 S 001-06
	Continuous Cell Line and Related JBI Products (continued)
	Cell Line Origin Comments Recommended Medium Related JBIProducts(Cat. No.)   GP + E-86  Mouse Based on NIH 3T3 fibroblasts. Ecotrophic retrovirual packaging cells. Gag and pol genes are on a different plasmid to the retroviral env gene. Transfection of these two plasmids created packaging cells with little chance of the packaging singnal from the disabled vector becoming associated with the helper virus replication.  DMEM  + 10% newborn calf serum LM 007-01 S 002-01   HeLa  Human Cervical carcinoma. Epithelioid, adherent, later rediagnosed as adenocarcinoma, widely used cell line. HeLa cells have been reported to contain human papilloma virus 18 (HPV-18) sequences.  MEM + NEAA + 10% FBS LM 007-01 LM 007-07 LS 005-01 S 001-01 S 001-03 S 001-06   HeLa 229  Human Derived from HeLa cells, resistant to polio virus. The cells are postive or keratin by immunoperoxide staining.  MEM + NEAA + 10% FBS LM 007-01 LM 007-07 LS 005-01 S 001-01 S 001-03 S 001-06   HeLa (Chang liver)  Human One of the first cell lines serially propagated from nonmalignant normal liver tissue. Extremaely useful in the study of malignancy. Adherent epithelial morphology.  BME + 10% FBS LM012-01 S 001-01 S 001-03 S 001-06   HeLa S3  Human Derived from HeLa cells; readily adaptable to growth in suspension; plate with 100% efficiency.HeLa cells have been reported to contain human papilloma virus 18 (HPV-18) sequences.  Ham's F-12K + 10% FBS LM 010-52 S 001-01 S 001-03 S 001-06   HEp-2  Human Derived from an epidermoid carcinoma of the larynx; supports the growth of 10 arboviruses and measies virus.  MEM + NEAA + 1 mM Na·Pyruvate + 10% FBS LM 007-01 LM 007-07 LS 005-01 LS 013-01 S 001-01 S 001-03 S 001-06   HepG2  Human Derived from an hepatocellular carcinoma. The cells express 3-hydroxy-3-methylglutaryl-CoA reductase and hepatic triglyceride lipase activities.  MEM + NEAA + 1 mM Na·Pyruvate + 10% FBS LM 007-01 LM 007-07 LS 005-01 LS 013-01 S 001-01 S 001-03 S 001-06   HL-60  Human Myeloid suspension cells derived from female patient with acute promyelocytic leukemia. HL-60 cells spontaneously differentiate and differentiation can be stimulated by butyrate, hypoxanthine, phorbol myristic acid (PMA, TPA), DMSO (1% to 5%), actinomycin D, and retinoic acid. The cells exhibit phagocytic activity and responsiveness to chemotactic stimuli. The line is positive for myc oncogene expression. Cells have been used for cloning myeloid cytokines.  RPMI 1640 + 10% FBS or IMDM + 20% FBS LM 011-01 LM 004-01 S 001-01 S 001-03 S 001-06   HSDM1C1  Mouse Subclone from cell line derived from Swiss albino mouse fibrosarcoma. Secretes prostaglandin E2; fibroblastic.  Ham's F-10 + 15% HS + 2.5% FBS LM 009-01 S 001-01 S 001-03 S 001-06 S 004-01   HT-29  Human Ultrastructural features include microvilli, microfilaments, large vacuolated mitochondria with dark granules, smooth and rough ER with free ribosomes, lipid droplets, few primary and many secondary lysosomes. HT-29 cells express urokinase receptors, but do not have detectable plasminogen activator activity.  McCoy's 5A + 10% FBS LM 005-01 S 001-01 S 001-03 S 001-06   IM-9  Human B-lymphoblast suspension cells isolated from bone marrow of female patient with multiple myeloma.IM-9 cell line has been shown to be an EBV-transformed B lymphoblastoid cell line. The cells are EBNA positive.  RPMI 1640 + 4.5 g/L glucose + 10 mM HEPES + 1 mM Na·Pyruvate + 10% FBS LM 011-01 LM 011-51 LS 001-02 BB 001-01 LS 013-01 S 001-01 S 001-03 S 001-06
	Continuous Cell Line and Related JBI Products (CONTINUED)
	Cell Line Origin Comments Recommended Medium Related JBIProducts(Cat. No.)   Jensen sarcoma  Rat Fibroblast-like rat sarcoma, requires aspargine, although independent variants occur at a rate of 1 : 10000. Cells require incubation in CO2 controlled incubator for only 24 hours after subculture.  McCoy's 5A + 5% FBS LM 005-01 S 001-01 S 001-03 S 001-06   Jurkat, Clone E6-1  Human The Jurkat cell line was established from the peripheral blood of a 14 year old boy. Clone E6-1 cells produce large amounts of IL-2 after stimulation with phorbol esters and either lectins or monoclonal antibodies against the T3 antigen.  RPMI 1640 + 4.5 g/L glucose +10 mM HEPES + 1 mM Na·Pyruvate + 10% FBS LM 011-01 LM 011-51 LS 001-02 BB 001-01 LS 013-01 S 001-01 S 001-03 S 001-06   K-562  Human Isolated from pleural effusion of a female patient in blast crises of chronic myelogenous leukemia. Subsequent studies show that, although Philadelphia chromosome positive, the cells demonstrate both myeloid and erythroid markers.  RPMI 1640 + 10% FBS LM 011-01 S 001-01 S 001-03 S 001-06   KB  Human Oral epidermoid carcinoma cells, early adherent monolayer culture. KB cells have been reported to contain human papillomavirus 18 (HPV-18P sequences.  MEM + NEAA + 1 mM Na·Pyruvate + 10% FBS LM 007-01 LM 007-07 LS 005-01 LS 013-01 S 001-01 S 001-03 S 001-06   KG-1  Human Male erythroleukemia patient, suspension myeloid cell, differentiate into macrophage-like cells after treatment with phorbol ester.  IMDM + 20% FBS LM 004-01 S 001-01 S 001-03 S 001-06   L 929  Mouse Connective tissue, commonly known as L cell. Fibroblast-like, one of the first cloned strains from one of the first cell strains established in continuous culture. Male. Popular choice in transfection studies.  MEM + NEAA + 10% FBS LM 007-01 LM 007-07 LS 005-01 S 001-01 S 001-03 S 001-06   LLC-MK2  Monkey Rhesus monkey kidney epithelial cells.  M199 + 1% HS +1.6 g/L NaHCO3 S 004-01   LLC-PK1  Pig Pig kidney epithelial cells, synthesize plasminogen activator.  M 199 + 3% FBS LM 006-01 S 001-01 S 001-03 S 001-06   LLC-RK1  Rabbit Rabbit kidney epithelial-like cells. Pooled New Zealand white animals, susceptible to viral infection (rubella, caccinia, herpes)  M 199 + 10% HS + 1.12g/L NaHCO3 S 004-01   LLC-WRC 256  Rat Highly malignant epithelial-like cells isolated from Walker rat carcinoma maintained in Harlan-Wistar rats. Supports propagation of herpes simplex and vaccinia virus.  M 199 + 5% HS LM 006-01S 004-01   L1210  Mouse Suspension cells from lymphocytic leukemia in DBA/2 mice. Chemical carcinogen induced, used for screening chemical agents for cytotoxicity.  DMEM + 10% HS  or   Fischer's medium + 10% HS LM 001-07 LM 014-01 S 004-01   MCF-7  Human Pleural effusion from female breast cancer post-radiation and hormone therapy, epithelial-like, adherent, characteristics of mammary epithelium.  MEM + NEAA + 10% FBS LM 007-01 LM 007-07 S 001-01 S 001-03 S 001-06   MDBK (NBL-1)  Bovine Bovine viral diarrhea free bovine kidney epithelial-like cells.  Ham's F-12 + 10% FBS LM 010-01 S 001-01 S 001-03 S 001-06   MDCK (NBL-2)  Dog Maden Darby canine kidney epithelial cells. Used for veterinary vaccine production. Respond to hepatocyte growth factor by tubule formation in collagen gels.  MEM + 10% FBS LM 007-01 S 001-01 S 001-03 S 001-06   MDBK (NBL-1)  Rat Epithelial-like buffalo rat hepatoma. Adherent, synthesizes and secretes rat serum albumin.  Ham's F-10 + 15% HS  + 2.5% FBS LM 009-01 S 004-01 S 001-01 S 001-03 S 001-06
	Continuous Cell Line and Related JBI Products (CONTINUED)
	Cell Line Origin Comments Recommended Medium Related JBIProducts(Cat. No.)   MiCl1 (S + L-)  Mink Mink lung cell line selected after Moloney murine sarcoma virus (MSV) infection. Contains an MSV genome rescuable by superinfection with a compatible helper virus. Used as a sensitive bioassay for helper virus. Adherent, fibroblast-like  RPMI 1640 + 10% FBS LM 011-01 S 001-01 S 001-03 S 001-06   MLA 144  Monkey Lymphoma derived from spontaneous gibbon lymphosarcoma. Constitutive release of gibbon IL-2, which is biologically active in both mouse and human systems. Also releases gibbon ape leukemia virus and should be handled accordingly.  RPMI 1640 + 10% FBS LM 011-01 S 001-01 S 001-03 S 001-06   Muntjac  Deer Indian male muntjac deer skin cell line. Primarily of interest because of the low chromosome number - 7 male, 8 female.  Ham's F-10 + 20% FBS LM 010-01 S 001-01 S 001-03 S 001-06   MOLT-3  Human This cell line was established from cells taken acute lymphoblastic leukemia (ALL) relapse patient. Suspension T cells. No EBV or Ig detectable. MOLT-3 was derived from the same patient as the MOLT-4 cell line.  RPMI 1640 + 4.5 g/L glucose +10 mM HEPES + 1 mM Na·Pyruvate + 10% FBS LM 011-01 LM 011-51 LS 001-02 BB 001-01 LS 013-01 S 001-01 S 001-03 S 001-06   MOLT-4  Human This cell line was established from cells taken acute lymphoblastic leukemia (ALL) relapse patient. Suspension T cells. No EBV or Ig detectable, able to resette with sheep red blood cells (SRBC), TdT activity.  RPMI 1640 + 10% FBS LM 011-01 S 001-01 S 001-03 S 001-06   MOPC-31C  Mouse Mouse, oil-induced plasmacytoma cell. Balb/c plasmacytoma suspension cell induced in animals by mineral oil injection. Secretes mg quatities of IgG1.  L-15 + 20% FBS LM 003-01 S 001-01 S 001-03 S 001-06   MPC-11  Mouse Derived from Merwin plasma cell tumor in Balb/c mice. Suspension myeloma cell line. Secretes Ig G2b  DMEM + 20% heat-inactivated HS LM 007-01 S 104-01   MRC-5  Human Derived from normal lung tissue of a 14-week-old male fetus. The cells are capable of 42 to 46 population doublings before the onset of senescence.  MEM + NEAA + 1 mM Na·Pyruvate + 10% FBS LM 007-01 LM 007-07 LS 005-01 LS 013-01 S 001-01 S 001-03 S 001-06   NB41A3  Mouse Subclone of adherent C-1300 mouse neuroblastoma cells. Resemble mature neurons and secrete neurotransmitter synthetic enzymes,  Ham's F-10 + 15% HS + 2.5% FBS LM 009-01 S 004-01 S 001-01 S 001-03 S 001-06   NCTC 2472  Mouse Connective tissue, high tumor producing, fibroblast-like, derived from NCTC 1742(VII). Produces tumors in 65% of C3H/HeN mice.  NCTC 135 + 10% HS S 004-01   NCTC 2555  Mouse Connective tissue, low tumor producing.  NCTC 135 + 10% HS S 004-01   Namalwa  Human Burkitt lymphoma. Suspension B cells. Single X chromosome, no Y.  RPMI 1640 + 10% FBS LM 011-01 S 001-01 S 001-03 S 001-06   Neuro-2a  Mouse Strain A albino mouse spontaneous neuroblastoma. Adherent, neuron and amoeboid-like. Produces large quantities of microtubular protein.  MEM + 10% FBS LM 007-01 S 001-01 S 001-03 S 001-06   NIH 3T3  Mouse Similar to 3T3 (Swiss) in origin, result of five serial cloning steps in order to isolate the clone most suitable for oncogenic transformation studies.  DMEM + 10% FBS LM 001-07 S 001-01 S 001-03 S 001-06   NRK-49F  Rat Normal rat kidney fibroblast. Contact inhibited, sensitive to transformation by viral or chemical agents. Cultures should be subcultured before they become confluent. If allowed to become confluent, the cells will transform.  DMEM + 5% FBS LM 001-07 S 001-01 S 001-03 S 001-06   NRK-52E  Rat Normal rat kidney epithelial-like cells. Distinct, but from the same source as NRK-49F.  DMEM + 5% FBS LM 001-07 S 001-01 S 001-03 S 001-06
	Continuous Cell Line and Related JBI Products (CONTINUED)
	< Cell Line Origin Comments Recommended Medium Related JBIProducts(Cat. No.)   P3/NSI/1-Ag4-1 (NS-1)  Mouse Abbreviation: NS-1Balb/c mouse myeloma clone. Nonimmunoglobulin-secreting clone of P3X63Ag8. Azaguanine (10-4 M) resistant, does not grow in HAT. Used as hybridoma fusion partner.  DMEM + 10% FBS LM 001-07 S 001-01 S 001-03 S 001-06   P3X63Ag8  Mouse Balb/c myeloma derived from P3K which was itself derived and established in culture from a Balb/c plasmacytoma (MPOC-21). Resistant to azaguanine, dies in HAT medium, parent of P3X63Ag8.635 and P3/NSI/1-Ag4-1 (NS-1)  DMEM + 10% FBS LM 001-07 S 001-01 S 001-03 S 001-06   P3X63Ag8.635  Mouse Balb/c myeloma subclone of P3X63Ag8. Has lost the ability to produce heavy or light-chain immunoglobulin. Azaguanine resistant and HAT sensitive, widely used as hybridoma fusion partner.  RPMI 1640 + 20% FBS LM 011-01 S 001-01 S 001-03 S 001-06   P388D1  Mouse Suspension chemically induced DBA/2 lymphoid neoplasm derived, monocyt-macrophage cell line. Subline P338D1(IL-1) secretes IL-1.  DMEM + 10% HS orFischer's medium + 10% HS LM 001-07 LM 014-01 S 004-01   P815  Mouse DBA/2 mastocytoma, suspension cells synthesize lysozyme and phagocytose latex beads, no ADCC effector activity, used as CTL target cells.  DMEM + 10% FBS LM 001-07 S 001-01 S 001-03 S 001-06   PA317  Mouse Derived from NIH/3T3 TK-, and transfected with plasmid-born (pPAM3) retroviral packaging constructs and HSV-TK. Allows the replication and secretion of infective, disabled, amphotrophic retrovrial particles. Virions produced by this line have been used successfully to transfer genes into humans. The percentage of PA317 cells that are capable of packaging retroviral vectors decreases slowly with continued passaging of the cell line, presumably due to the loss of the transfected DNA used to create the line.  DMEM + 10% FBS LM 001-07 S 001-01 S 001-03 S 001-06   Raji  Human Burkitt lymphoma suspension B cells, male, EBNA positive, c-myc genen translocation and mutations.  RPMI 1640 + 10% FBS LM 011-01 S 001-01 S 001-03 S 001-06   RPMI 1788  Human EBNA-positive hematopoietic cells, isolated from the peripheral blood of an apparently normal male. Mammalian and Insect cell culture 동물 세포 배양의 역사 세포배양시 필요한 장치 생세포수의 계산 살균용 ethanol의 농도 부착 세포 배양과 부유 세포 배양 세포의 동결 보존 동결 보존된 세포의 배양 세포의 계대배양 세포주(continuous cell lines) 곤충 세포의 배양 Classical Media Classical Media 종류별 성분 비교 세포 배양 배지의 제조에 사용하는 초순수물 Classical Media 성분 Hanks' salt와 Earle's salt의 차이점 세포 배양 배지에 적용되는 완충 작용 L-Glutamine의 함량이 다른 아미노산보다 높은 이유? L-Alanyl-L-Glutamine과 L-Glutamine 무균 시험(steriliy test) 내독소 시험(endotoxin test) Serum-Free and Protein Free Media Serum-free media와 protein-free media Serum-free media와 protein-free media의 장점 Serum-free media와 protein-free media의 단점 Serum-free media 또는 protein-free media를 PBMC의 배양 Serum-free hybridoma media 또는 protein-free hybridoma media�.. Serum-Free CHO media 또는 protein-free CHO media로의 적응 .. 혈청 대체 물질 (serum replacements) Supplements 세포 배양에서 혈청의 역할 혈청의 종류 혈청의 성분 혈청의 열불활성화 (heat-inactivation) 혈청의 저장 혈청의 해동 방법 세포 배양시 사용하는 항생제 Geneticin(G418 sulfate) 호르몬(hormone) Soybean trypsin inhibitor 성장 인자(growth factors)