1 kb Ladder

Catalog Number DM 1027, 1028, 1027L, 1028L

Storage Temperature -20ºC

Product Description

The 1kb Ladder is prepared from WelGENE recombinant plasmids digested by adequate restriction enzymes, followed by phenol inactivation of enzyme. And other materials are also completely removed. So you can always see the bands with high resolution and the same band pattern. The 1 kb Ladder contains 10 double stranded DNA fragments ranging in size from 1~10 kb in 1 kb increment and one 500 bp DNA fragment. Especially, concentration of 2 kb and 5 kb DNA bands are 2~3 times higher than other bands, so you can more easily estimate your sample size.

Storage Buffer (for DM 1027, DM 1028)

12 mM Tris-HCl (pH 8.0)

1 mM EDTA (pH 8.0)

6X Loading Dye (for DM 1027, DM 1028)

15 % (w/v) Ficoll

0.05 % (w/v) Bromophenol blue

45 mM EDTA (pH 8.0)

Buffer I (for DM 1027L, DM 1028L)

10 mM Tris-HCl (pH 8.0)

8.33 mM EDTA (pH 8.0)

2.5 % (w/v) Ficoll

0.0083 % Bromophenol blue

Final concentration (when mixed with lyophilized DNA)

2.5 % (w/v) Ficoll

7.5 mM EDTA (pH 8.0)

Instructions For Use

Solution Form

Mix DNA solution dissolved in TE buffer (pH 8.0) with 6X Loading Dye offered with our product in the ratio of 5:1 before use.

Ex. 1) 5 ul of DNA solution + 1 ul of 6X Loading Dye

Ex. 2) 10 ul of DNA solution + 2 ul of 6X Loading Dye

Lyophilized Form

Add 600 ul (DM 1027L), or 1.2 ml (DM 1028L) of Buffer I solution offered with our product to blue DNA precipitate, and dissolve it completely so that it may become the concentration indicated at the bottom of this product information just before using it.

*Once dissolved, store at less than 0ºC

Precautions

For In Vitro Use Only.