1 M Tris-HCl Buffer Solutions

1 M Tris-HCl buffer is a versatile buffer solution used for a wide variety of purposes in molecular biology, such as the production of Tris-EDTA (TE) buffer. Tris {tris(hydroxymethyl)aminomethane; 2-amino-2-(hydroxymethyl)-1,3-propanediol; trometh-amine; trometamol; C₄H₁₁NO₃, FW 121.1} is a representative buffer solution appropriate for a vast majority of enzyme reactions and biochemical experiments. Tris, which is highly soluble, exhibits a pKa of 8.1 (25°C) and buffers at maximum efficiency between pH ranges of 7.0 – 9.1. When the pH goes below 7.0 or above 9.1, however, the product’s buffering capability diminishes and the pH becomes susceptible to temperature (1°C increase leads to 0.03 drop in pH). For accurate pH measurements, a specialized pH probe for Tris solutions is required. The product is highly reactive and cannot be used with glutaraldehyde, formaldehyde, and glyoxal. Some animal cells also show toxic responses to the product; therefore such facts should be considered in advance to using the product.

To dilute WelGENE’s 1 M Tris-HCl buffer, Ultra Pure Water (ML 019-02) is to be used.