[NOTICE] 웰진 웹사이트의 만족도를 조사합니다.
Cart0
[Bulk] DMEM / F-12 1:1 Mixture (LM002-04)

[Bulk] DMEM / F-12 1:1 Mixture (LM002-04)


  오후 1시 이전 주문은 당일 발송됩니다.
  냉장, 냉동 제품은 금요일에는 발송이 불가능하며, 차주 월요일에 발송됩니다.
  재고 소진 시 배송이 지연 될 수 있습니다.(제품마다 생산소요기간 상이)
▣ 대량 구매시 할인 적용 제품입니다.
1. 20병 이상 구매 시 보관 및 분할 배송 서비스 가능합니다.
2. 분할 배송을 원하시면 본사로 문의바랍니다.(1년이내 전량소진, 최소 출고수량: 5병 이상 / 1회당)
QTY 1-19 20-49 50-99 100+
500ML ₩23,800 ₩22,700 ₩21,500 ₩20300
Size
Price
Quantity
500ML X 20
₩454,000
500ML X 50
₩1,075,000
500ML X 100
₩2,030,000
 
Special Order for bulk

Contact Us
Product Details

제품 설명서 (PDF 파일 다운로드) MSDS (PDF 파일 다운로드)

 

Dulbecco’s Modified Eagle’s Medium / Nutrient Mixture F-12 (DMEM/F-12) 1:1 Mixture (1X), Liquid
With 15mM HEPES
With L-glutamine

Catalog Number LM 002-04
                           LM 002-05 (without phenol red)
                           LM 002-06 (without sodium bicarbonate)
Storage Temperature 2~8°C

 Product Description
F-12 Nutrient Mixture (Ham’s F-12, LM 010-02) is used for serum-free cultivation of cell lines. During the past decade, researchers have reported the culture of Leydig cells and Sertoli cells in serum-free medium that contained insulin, transferrin, epidermal growth factor (EGF), leutinizing hormone or follicle stimulating hormone, somatomedin and growth hormone. A 1:1 mixture of Dulbecco’s Modified Eagle’s Medium (DMEM) and Ham’s F-12 Nutrient Mixture is found to be most satisfactory for studies of this type.

LM 002-04, LM 002-05 and LM 002-06 contain, instead of serum, a supplement of nutrients, growth factors, hormones, and 365 mg/L L-glutamine. HEPES buffer is included in the formulation at a final concentration of 15mM to compensate for the loss of buffering capacity incurred by eliminating serum.

LM 002-05 does not contain phenol red, and LM 002-06 does not contain sodium bicarbonate. The selection of a nutrient medium is strongly influenced by (1) type of cell, (2) type of culture (monolayer, suspension, or clonal) and (3) degree of chemical definition necessary. It is important to review the literature for recommendations concerning medium, supplementation and physiological parameters required for a specific cell line.

Storage/Stability
The liquid medium should be stored at 2~8°C in the dark. Deterioration of the liquid medium may be recognized by (1) precipitate or particulate matter throughout the solution, (2) cloudy appearance, (3) color change, and/or (4) pH change. The nature of supplements added may affect storage conditions and shelf life of the medium. Product label bears expiration date.

Biological Performance Characteristics
The growth-promoting capacities of DMEM/F-12 medium are tested in a medium using an appropriate cell line(s). Growth rates are examined through three subculture generations and compared with parallel cultures grown in standardized control medium. Cells are counted and growth is plotted as a logarithmic function of time in culture, and seeding efficiency, doubling time, and final cell densities are determined. During the testing period cultures are examined microscopically for a typical morphology and evidence of cytotoxicity.

Precautions
For In Vitro Use Only

 

* In effort to cater to the individual needs of our clients, Welgene provides custom products (classified as a “special order”) with variations made to the general specifications listed on the catalogue. Variations can be made in regards to 1. elimination of certain materials 2. alteration of chemical portions 3. addition of new materials and 4. change in pH.

Comparison of liquid DMEM/F-12, 1:1 Mixtures

Comparison of special ordered liquid DMEM/F-12, 1:1 Mixtures 

* In effort to cater to the individual needs of our clients, Welgene provides custom products (classified as a “special order”) with variations made to the general specifications listed on the catalogue. Variations can be made in regards to 1. elimination of certain materials 2. alteration of chemical portions 3. addition of new materials and 4. change in Ph.