McCoy’s 5A Medium, modified (1X) Liquid
냉장, 냉동 제품은 금요일에는 발송이 불가능하며, 차주 월요일에 발송됩니다.
재고 소진 시 배송이 지연 될 수 있습니다.(제품마다 생산소요기간 상이)
McCoy’s 5A Medium, modified (1X) Liquid
With L-glutamine
Catalog Number LM 005-01 (without HEPES)
LM 005-02 (With HEPES)
Storage Temperature 2~8°C
Product Description
McCoy and his coworkers developed McCoy’s 5A Medium for in vitro cultivation of Novikoff Hepatoma Cells. Basal Medium 5A was used and modified for these studies to support Walker Carcinosarcoma 256 Cells. In this medium, Hsu and Kellogg cultured successfully primary cultures derived from normal bone marrow, skin, gingiva, testes, mouse kidney, omentum, adrenal glands, lung, spleen, rat embryos, and other tissues.
LM 005-01 and LM 005-02 contain 219.2 mg/L Lglutamine. HEPES buffer is included in LM 005-02 at the concentration of 25 mM. The selection of a nutrient medium is strongly influenced by (1) type of cell, (2) type of culture (monolayer, suspension, or clonal) and (3) degree of chemical definition necessary. It is important to review the literature for recommendations concerning medium, supplementation and physiological parameters required for a specific cell line.
Storage/Stability
The liquid McCoy’s 5A medium should be stored at 2~8°C in the dark. Deterioration of the liquid medium may be recognized by (1) precipitate or particulate matter throughout the solution, (2) cloudy appearance, (3) color change, and/or (4) pH change. The nature of supplements added may affect storage conditions and shelf life of the medium. Product label bears expiration date.
Biological Performance Characteristics
The growth-promoting capacities of McCoy’s 5A medium are tested in a medium containing 10% FBS using an appropriate cell line(s). Growth rates are examined through three subculture generations and compared with parallel cultures grown in standardized control medium. Cells are counted and growth is plotted as a logarithmic function of time in culture, and seeding efficiency, doubling time, and the final cell density are determined. During the testing period cultures are examined microscopically for a typical morphology and evidence of cytotoxicity.
Precautions
For In Vitro Use Only
Components |
LM 005-01 |
LM 005-02 |
CaCl2 (anhydrous) |
100.00 |
100.00 |
KCl |
400.00 |
400.00 |
MgSO4 (anhydrous) |
98.00 |
98.00 |
NaCl |
5100.00 |
5100.00 |
NaHCO3 |
2200.00 |
2200.00 |
NaH2PO4·H2O |
580.00 |
580.00 |
Bacto-peptone 600 |
600.00 |
600.00 |
HEPES |
- |
5958.00 |
D-Glucose |
3000.00 |
3000.00 |
Glutathione (reduced) |
0.50 |
0.50 |
Phenol Red |
10.00 |
10.00 |
L-Alanine |
13.90 |
13.90 |
L-Arginine·HCl |
42.10 |
42.10 |
L-Asparagine |
45.00 |
45.00 |
L-Aspartic Acid |
20.00 |
20.00 |
L-Cysteine |
31.50 |
31.50 |
Glycine |
7.50 |
7.50 |
L-Glutamic Acid |
22.10 |
22.10 |
L-Glutamine |
219.20 |
219.20 |
L-Histidine·HCl·H2O |
21.00 |
21.00 |
L-Hydroxyproline |
19.70 |
19.70 |
L-Isoleucine |
39.40 |
39.40 |
L-Leucine |
39.40 |
39.40 |
L-Lysine·HCl |
36.50 |
36.50 |
L-Methionine |
15.00 |
15.00 |
L-Phenylalanine |
16.50 |
16.50 |
L-Proline |
17.30 |
17.30 |
L-Serine |
26.30 |
26.30 |
L-Threonine |
17.90 |
17.90 |
L-Tryptophan |
3.10 |
3.10 |
L-Tyrosine·2Na·2H2O |
26.20 |
26.20 |
L-Valine |
17.60 |
17.60 |
Asxorbic Acid |
0.50 |
0.50 |
Biotin |
0.20 |
0.20 |
Choline Chloride |
5.00 |
5.00 |
D-Ca Panto thenate |
0.20 |
0.20 |
Folic Acid |
10.00 |
10.00 |
i-Inositol |
36.00 |
36.00 |
Niacinamide |
0.50 |
0.50 |
Nicotinic Acid |
0.50 |
0.50 |
Para-aminobenzoic Acid |
1.00 |
1.00 |
Pyridoxal·HCl |
0.50 |
0.50 |
Pyridoxine·HCl |
0.50 |
0.50 |
Riboflavin |
0.20 |
0.20 |
Thiamine·HCl |
0.20 |
0.20 |
Vitamin B12 |
2.00 |
2.00 |
Product Profile |
LM 005-01 |
LM 005-02 |
Appearance |
Red transparent solution |
Red transparent solution |
pH at RT |
7.0 ~ 7.6 |
7.0 ~ 7.6 |
Osmolality |
272 ~ 300 mOsm/kg H2O |
315 ~ 349 mOsm/kg H2O |
Endotoxin |
£ 1.0 EU/ml |
£ 1.0 EU/ml |
Sterility |
Sterilized by 0.2 mm filtration system. Sterility tests are performed in accordance with protocols described in USP. |
References
McCoy, T.A., Maxwell, M. and Kruse, P.F., (1959). Amino Acid Requirement of the Novioff Hepatoma In Vitro. Proc. Soc. Exp. Biol. Med. 100, 115-118.
Patterson, M.K. and Dell’orco, R.T., (1978). Preparation of McCoy’s Medium 5A. Tissue Culture Association Manual. 4, 737-740.
Hsu, T.C. and Kellogg, D.S., (1960). Primary Cultivation and Continuous Propagation In Vitro of Tissues from Small Biopsy Specimens. J.N.C.I. 25, 221-231.
Iwakata, S. and Grace, J.T., (1964). Cultivation in Vitro of Myeoblasts from Human Leukemia. New York State Journal of Med. 64, 2279-2282.
Morton, H.J., (1970). A Survey of Commercially Available Tissue Culture Media. In Vitro. 6, 89.