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McCoy’s 5A Medium, modified (1X) Liquid

McCoy’s 5A Medium, modified (1X) Liquid


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500ml
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1L
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Product Details

McCoy’s 5A Medium, modified (1X) Liquid
With L-glutamine

Catalog Number LM 005-01 (without HEPES)
                           LM 005-02 (With HEPES)
Storage Temperature 2~8°C

Product Description
McCoy and his coworkers developed McCoy’s 5A Medium for in vitro cultivation of Novikoff Hepatoma Cells. Basal Medium 5A was used and modified for these studies to support Walker Carcinosarcoma 256 Cells. In this medium, Hsu and Kellogg cultured successfully primary cultures derived from normal bone marrow, skin, gingiva, testes, mouse kidney, omentum, adrenal glands, lung, spleen, rat embryos, and other tissues.

LM 005-01 and LM 005-02 contain 219.2 mg/L Lglutamine. HEPES buffer is included in LM 005-02 at the concentration of 25 mM. The selection of a nutrient medium is strongly influenced by (1) type of cell, (2) type of culture (monolayer, suspension, or clonal) and (3) degree of chemical definition necessary. It is important to review the literature for recommendations concerning medium, supplementation and physiological parameters required for a specific cell line.

Storage/Stability
The liquid McCoy’s 5A medium should be stored at 2~8°C in the dark. Deterioration of the liquid medium may be recognized by (1) precipitate or particulate matter throughout the solution, (2) cloudy appearance, (3) color change, and/or (4) pH change. The nature of supplements added may affect storage conditions and shelf life of the medium. Product label bears expiration date.

Biological Performance Characteristics
The growth-promoting capacities of McCoy’s 5A medium are tested in a medium containing 10% FBS using an appropriate cell line(s). Growth rates are examined through three subculture generations and compared with parallel cultures grown in standardized control medium. Cells are counted and growth is plotted as a logarithmic function of time in culture, and seeding efficiency, doubling time, and the final cell density are determined. During the testing period cultures are examined microscopically for a typical morphology and evidence of cytotoxicity.

Precautions
For In Vitro Use Only


 

Components

LM 005-01

LM 005-02

CaCl2 (anhydrous)

100.00

100.00

KCl

400.00

400.00

MgSO4 (anhydrous)

98.00

98.00

NaCl

5100.00

5100.00

NaHCO3

2200.00

2200.00

NaH2PO4·H2O

580.00

580.00

Bacto-peptone 600

600.00

600.00

HEPES

-

5958.00

D-Glucose

3000.00

3000.00

Glutathione (reduced)

0.50

0.50

Phenol Red

10.00

10.00

L-Alanine

13.90

13.90

L-Arginine·HCl

42.10

42.10

L-Asparagine

45.00

45.00

L-Aspartic Acid

20.00

20.00

L-Cysteine

31.50

31.50

Glycine

7.50

7.50

L-Glutamic Acid

22.10

22.10

L-Glutamine

219.20

219.20

L-Histidine·HCl·H2O

21.00

21.00

L-Hydroxyproline

19.70

19.70

L-Isoleucine

39.40

39.40

L-Leucine

39.40

39.40

L-Lysine·HCl

36.50

36.50

L-Methionine

15.00

15.00

L-Phenylalanine

16.50

16.50

L-Proline

17.30

17.30

L-Serine

26.30

26.30

L-Threonine

17.90

17.90

L-Tryptophan

3.10

3.10

L-Tyrosine·2Na·2H2O

26.20

26.20

L-Valine

17.60

17.60

Asxorbic Acid

0.50

0.50

Biotin

0.20

0.20

Choline Chloride

5.00

5.00

D-Ca Panto thenate

0.20

0.20

Folic Acid

10.00

10.00

i-Inositol

36.00

36.00

Niacinamide

0.50

0.50

Nicotinic Acid

0.50

0.50

Para-aminobenzoic Acid

1.00

1.00

Pyridoxal·HCl

0.50

0.50

Pyridoxine·HCl

0.50

0.50

Riboflavin

0.20

0.20

Thiamine·HCl

0.20

0.20

Vitamin B12

2.00

2.00

 

Product

Profile

LM 005-01

LM 005-02

Appearance

Red transparent solution

Red transparent solution

pH at RT

7.0 ~ 7.6

7.0 ~ 7.6

Osmolality

272 ~ 300 mOsm/kg H2O

315 ~ 349 mOsm/kg H2O

Endotoxin

£ 1.0 EU/ml

£ 1.0 EU/ml

Sterility

Sterilized by 0.2 mm filtration system. Sterility tests are performed in accordance with protocols described in USP.

 

 

References

McCoy, T.A., Maxwell, M. and Kruse, P.F., (1959). Amino Acid Requirement of the Novioff Hepatoma In Vitro. Proc. Soc. Exp. Biol. Med. 100, 115-118.
Patterson, M.K. and Dell’orco, R.T., (1978). Preparation of McCoy’s Medium 5A. Tissue Culture Association Manual. 4, 737-740.
Hsu, T.C. and Kellogg, D.S., (1960). Primary Cultivation and Continuous Propagation In Vitro of Tissues from Small Biopsy Specimens. J.N.C.I. 25, 221-231.
Iwakata, S. and Grace, J.T., (1964). Cultivation in Vitro of Myeoblasts from Human Leukemia. New York State Journal of Med. 64, 2279-2282.
Morton, H.J., (1970). A Survey of Commercially Available Tissue Culture Media. In Vitro. 6, 89.