Protein-Free Hybridoma Medium, Liquid

With D-glucose

With sodium bicarbonate

With L-glutamine

Without HEPES

For the adherent culture of Hybridoma cell

Catalog Number PF 301

Storage Temperature 2~8°C

Product Description

Protein-Free Hybridoma Media are liquid media formulated to support suspension culture of hybridoma cells. Protein-Free Hybridoma Media excludes protein from its formulation. Protein-Free Hybridoma Media differs from normal (serum supplemented) media in its amino acid, vitamin, and mineral composition, and includes peptides, fatty acids, and growth factors as substitutes to serum. Hybridoma cells that had been formerly cultured in serum supplemented media normally require adjustment period in order to successfully propagate in Protein-Free Hybridoma Medium. Adjustment periods can be shortened when adjustment methods are properly administered.

PF 301 contains D-glucose, sodium bicarbonate, L-glutamine, but not includes HEPES. Therefore requires 1 M HEPES (BB 001-01) supplements when needed. PF 301 vary in their compositions and consequently yield different effects on different cell lines. The optimal product for a certain cell type can only be confirmed through direct application. PF 301 contains no ingredients of animal origin, and is therefore appropriate in the research and production of medical grade recombinant proteins. In selecting the appropriate culture environment of Protein-Free Medium, (1) the origin of the cell host (the kind of cell), (2) the type of culture container to be used (tissue flask, multiplayer flasks, roller bottle, spinner flasks, or bioreactors), and (3) the characteristics of the cell host, are important factors to be considered.

Storage/Stability

The Protein-Free medium should be stored at 2~8°C in the dark. Deterioration of the liquid medium may be recognized by (1) precipitate or particulate matter throughout the solution, (2) cloudy appearance, (3) color change, and/or (4) pH change. The nature of supplements added may affect storage conditions and shelf life of the medium. Product label bears expiration date.

Instructions for Use

(1) Nutritional requirements can change in accordance with each gene expression system, (2) recombinant protein expression can be increased by supplementing polyamine supplement (LS 018-01), fatty acid supplement (LS 017-01), sodium butyrate (LS 033-01), (3) when using a CO₂ incubator, the pH of the cell culture should be adjusted by controlling the NaHCO₃ concentration, (4) when using a spinner flask, leave the cover 1/4 open to allow aeration.

Biological Performance Characteristics

The growth-promoting capacities of the Protein-Free Hybridoma media are tested in a liquid medium using Hybridoma cell. Growth rates are examined through three subculture. Cells are counted and growth is plotted as a logarithmic function of time in culture, and seeding efficiency, doubling time, and final cell densities are determined. During the testing period cultures are examined microscopically for a typical morphology and evidence of cytotoxicity.

Precautions

Protein-Free Hybridoma media are only used In Vitro, and are sensitive to light and heat. Adhering to the following suggestions can increase the product shelf life. (1) Limit light exposure, (2) minimize its exposure to room temperature (3) store in a darkroom at 2~8°C, (4) use in small amounts at a time.