MCDB 302 Medium
With L-glutamine
With sodium bicarbonate
Without nucleosides
Catalog Number LM 016-07
Storage Temperature 2~8°C
Product Description
MCDB Media were initially formulated to support low-protein or serum-free growth of specific cell types. Each MCDB medium is customized for optimally balanced nutritional environment that selectively promoted growth of specific cell. MCDB 302 medium is variations of the F-12 nutrient mixture Ham (Ham`s F-12) medium, appropriate for clonal growth of Chinese hamster ovary (CHO) cells.
LM 016-07 contains the 438.60 mg/L L-glutamine and 1180.00 mg/L sodium bicarbonate. The selection of a nutrient medium is strongly influenced by (1) type of cell, (2) type of culture (monolayer, suspension, clonal), and (3) degree of chemical definition necessary. It is important to review the literature for recommendations concerning medium, supplementation and physiological parameters required for a specific cell line.
Storage/Stability
The liquid medium should be stored at 2~8°C in the dark. Deterioration of the liquid medium may be recognized by (1) precipitate or particulate matter throughout the solution, (2) cloudy appearance, (3) color change, and/or (4) pH change. The nature of supplements added may affect storage conditions and shelf life of the medium. Product label bears expiration date.
Biological Performance Characteristics
The growth-promoting capacities of the liquid media are tested in a medium containing 10% FBS using an appropriate cell line(s). Growth rates are examined through three subculture generations and compared with parallel cultures grown in standardized control medium. Cells are counted and growth is plotted as a logarithmic function of time in culture, and seeding efficiency, doubling time, and final cell densities are determined. During the testing period cultures are examined microscopically for a typical morphology and evidence of cytotoxicity.
Precautions
For In Vitro Use Only
| Product Name |
Description |
Cat. No. |
Recommended Storage Temp. |
Size |
|
MCDB 105 Medium Complete
with trace elements, liquid
|
For long term growth of human diploid fibroblast-like cells
With L-glutamine
With 25 mM HEPES
Without sodium bicarbonate |
LM 016-01 |
Store at 2-8°C |
500ML, 1L |
MCDB 131 Medium Complete
with trace elements, liquid |
For clonal growth of human microvascular endothelial cells (HMVEC)
With L-glutamine
With sodium bicarbonate |
LM 016-03 |
Store at 2-8°C |
500ML, 1L |
MCDB 151 Medium Complete
with trace elements, liquid |
For growth of primary human keratinocytes
With L-glutamine
With 28 mM HEPES
With sodium bicarbonate |
LM 016-04 |
Store at 2-8°C |
500ML, 1L |
MCDB 153 Medium Complete
with trace elements, liquid |
For clonal and long term growth of human epidermal keratinocytes
With L-glutamine
With 28 mM HEPES
With sodium bicarbonate |
LM 016-05 |
Store at 2-8°C |
500ML, 1L |
MCDB 201 Medium Complete
with trace elements, liquid |
For clonal growth of chicken embryo fibroblasts
With L-glutamine
With 30 mM HEPES
Without sodium bicarbonate |
LM 016-06 |
Store at 2-8°C |
500ML, 1L |
MCDB 302 Medium Complete
with trace elements, liquid |
For clonal growth of Chinese hamster ovary (CHO) cells
With L-glutamine
With sodium bicarbonate
Without nucleosides |
LM 016-07 |
Store at 2-8°C |
500ML, 1L |
* In effort to cater to the individual needs of our clients, Welgene provides custom products (classified as a “special order”) with variations made to the general specifications listed on the catalogue. Variations can be made in regards to 1. elimination of certain materials 2. alteration of chemical portions 3. addition of new materials and 4. change in pH.
Comparison of liquid MCDB Media
| Product Name |
Cat. No. |
L-Glutamine (mg/L) |
HEPES (mg/L) |
Sodium Bicarbonate (mg/L) |
| MCDB 105 |
LM 016-01 |
365.30 (2.5 mM) |
5958.00 (25 mM) |
- |
| MCDB 131 |
LM 016-03 |
1461.00 (10.0 mM) |
- |
1180.00 |
| MCDB 151 |
LM 016-04 |
877.20 (6.0 mM) |
6600.00 (28 mM) |
1180.00 |
| MCDB 153 |
LM 016-05 |
877.20 (6.0 mM) |
6600.00 (28 mM) |
1200.00 |
| MCDB 201 |
LM 016-06 |
146.15 (1.0 mM) |
7149.00 (30 mM) |
- |
| MCDB 302 |
LM 016-07 |
438.60 (3.0 mM) |
- |
1180.00 |
Applications of some MCDB Media
| Cell Type |
MCDB Medium |
Additions |
| Endothelial Cell |
MCDB 131 |
Hydrocortisone : 1 μg/ml
Bovine Brain Extract : 12 μg/ml
EGF : 10 ng/ml
|
| Keratinocyte |
MCDB 153 |
Hydrocortisone : 0.5 μg/ml
Insulin : 5 μg/ml
Bovine Pituitary Extract : 30 μg/ml
EGF : 0.1 ng/ml
|
| Melanocyte |
MCDB 153 |
Hydrocortisone : 0.5 μg/ml
Insulin : 5 μg/ml
Bovine Pituitary Extract : 26 μg/ml
Fibroblast Growth Factor : 1 ng/ml
Phorbol Ester : 10 ng/ml
|
| Chinese hamster ovary (CHO) cell |
MCDB 302 |
Insulin
Transferrin
Methylcellulose |
