Dulbecco's Modified Eagle's Medium (DMEM) (1X), Liquid (Low Glucose)

With 1000 mg/L D-glucose
Without L-glutamine

Catalog Number LM 001-02
Storage Temperature 2~8°C

Product Description

For animal cell culture, Eagle’s Medium have been developed and modified since the original formulation appeared in the literature. Dulbecco’s Modified Eagle’s Medium (DMEM) is a modification of Minimum Essential Medium (MEM) that contains a higher concentration of amino acids and vitamins, as well as additional supplementary components, such as D-glucose (low or high, 1000 mg/L or 4500 mg/L, respectively).

LM 001-02 contains 1000 mg/L D-glucose, but no L-glutamine. Add aseptically 20 mL of sterile 200 mM L-glutamine solution (LS 002-01) per 1 L of medium before use. The selection of a nutrient medium is strongly influenced by (1) type of cell, (2) type of culture (monolayer, suspension, clonal), and (3) degree of chemical definition necessary. It is important to review the literature for recommendations concerning medium, supplementation and physiological parameters required for a specific cell line.

Storage/Stability

The liquid medium should be stored at 2~8°C in the dark. Deterioration of the liquid medium may be recognized by (1) precipitate or particulate matter throughout the solution, (2) cloudy appearance, (3) color change, and/or (4) pH change. The nature of supplements added may affect storage conditions and shelf life of the medium. Product label bears expiration date.

Biological Performance Characteristics

The growth-promoting capacities of DMEM are tested in a medium containing 10% FBS using an appropriate cell line(s). Growth rates are examined through three subculture generations and compared with parallel cultures grown in standardized control medium. Cells are counted and growth is plotted as a logarithmic function of time in culture, and seeding efficiency, doubling time, and final cell densities are determined. During the testing period cultures are examined microscopically for a typical morphology and evidence of cytotoxicity.

Precautions
For In Vitro Use Only