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1 M Tris-HCl (pH 6.9), (ML017-69)

1 M Tris-HCl (pH 6.9), (ML017-69)

  오후 1시 이전 주문은 당일 발송됩니다.
  냉장, 냉동 제품은 금요일에는 발송이 불가능하며, 차주 월요일에 발송됩니다.
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1 M Tris-HCl Buffer Solutions

1 M Tris-HCl buffer is a versatile buffer solution used for a wide variety of purposes in molecular biology, such as the production of Tris-EDTA (TE) buffer. Tris {tris(hydroxymethyl)aminomethane; 2-amino-2-(hydroxymethyl)-1,3-propanediol; trometh-amine; trometamol; C4H11NO3, FW 121.1} is a representative buffer solution appropriate for a vast majority of enzyme reactions and biochemical experiments. Tris, which is highly soluble, exhibits a pKa of 8.1 (25°C) and buffers at maximum efficiency between pH ranges of 7.0 – 9.1. When the pH goes below 7.0 or above 9.1, however, the product’s buffering capability diminishes and the pH becomes susceptible to temperature (1°C increase leads to 0.03 drop in pH). For accurate pH measurements, a specialized pH probe for Tris solutions is required. The product is highly reactive and cannot be used with glutaraldehyde, formaldehyde, and glyoxal. Some animal cells also show toxic responses to the product; therefore such facts should be considered in advance to using the product.

To dilute WelGENE’s 1 M Tris-HCl buffer, Ultra Pure Water (ML 019-02) is to be used.