40%T, 3.3%C Acrylamide/Bis-Acrylamide Solution (29:1)
Contains 386.8 g/L acrylamide and
13.2 g/L bis-acrylamide in Ultra Pure Water
Sterile-filtered
DNase, RNase and protease-none detected

Catalog Number ML 002-05
Storage Temperature 2~8°C

Product Description
Acrylamide/N,N`-methylenebisacrylamide (Acrylamide/bis- acrylamide) solution is an essential ingredient in making poly acrylamide gel, which is used in electrophoresis of protein or nucleic acid. In the present product, acrylamide and bis-acrylamide are pre-dissolved at various concentrations for customer convenience and safety. The gel matrix is formed by free radical polymerization of acrylamide and comonomer crosslinker (bis-acrylamide).

The gel pores size is determined by two parameters:

• Total monomer concentration (%T)
• The weight percentage of crosslinker (%C)

%T = {acrylamide (g) + bis-acrylamide (g)} / volumn (ml) x 100

%C = bis-acrylamide (g) / {acrylamide (g) + bis-acrylamide (g)} x 100

Gel with T=20% is prepared with 20% of acrylamide and bis-acrylamide. As %T is higher, the pores size of the gel are smaller. Gel with T=20%, C=5% is prepared with 20% of acrylamide and bis-acrylamide and the weight percentage of the bis-acrylamide is 5% from the total weight of the acrylamide and bis-acrylamide. Welgene provides two different concentrations for acrylamide/bis-acrylamide solutions (30% and 40%), and three different cross-linking ratios exist for each concentrations percentage (19:1, 29:1, and 37.5:1).

ML 002-05 contains 386.8 g/L acrylamide and 13.2 g/L bis-acrylamide in ultra pure water.

Storage/Stability
Acrylamide/bis-acrylamide solution should be stored at 2~8°C. And acrylamide/bis-acrylamide solution needs to be stored away from light. Acrylamide and bis-acrylamide slowly change into acrylic acid and bis-acrylic acid, respectively, over long periods of time. Deterioration of the solution may be recognized by (1) precipitate or particulate matter throughout the solution, (2) cloudy appearance, (3) color change, and/or (4) pH change. Product label bears expiration date.

Biological Performance Characteristics
The biological characteristics of the acrylamide/bis-acrylamide solution are tested using gel electrophoresis of protein or nucleic acid, and compared with the resolution of the parallel bands in standardized control solution.

Precautions
For In Vitro Use Only