McCoy’s 5A Medium, modified (1X) Liquid

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Size

Price

Quantity

500ml

₩0

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Product Details

McCoy’s 5A Medium, modified (1X) Liquid
With L-glutamine

Catalog Number LM 005-01 (without HEPES)
LM 005-02 (With HEPES)
Storage Temperature 2~8°C

Product Description
McCoy and his coworkers developed McCoy’s 5A Medium for in vitro cultivation of Novikoff Hepatoma Cells. Basal Medium 5A was used and modified for these studies to support Walker Carcinosarcoma 256 Cells. In this medium, Hsu and Kellogg cultured successfully primary cultures derived from normal bone marrow, skin, gingiva, testes, mouse kidney, omentum, adrenal glands, lung, spleen, rat embryos, and other tissues.

LM 005-01 and LM 005-02 contain 219.2 mg/L Lglutamine. HEPES buffer is included in LM 005-02 at the concentration of 25 mM. The selection of a nutrient medium is strongly influenced by (1) type of cell, (2) type of culture (monolayer, suspension, or clonal) and (3) degree of chemical definition necessary. It is important to review the literature for recommendations concerning medium, supplementation and physiological parameters required for a specific cell line.

Storage/Stability
The liquid McCoy’s 5A medium should be stored at 2~8°C in the dark. Deterioration of the liquid medium may be recognized by (1) precipitate or particulate matter throughout the solution, (2) cloudy appearance, (3) color change, and/or (4) pH change. The nature of supplements added may affect storage conditions and shelf life of the medium. Product label bears expiration date.

Biological Performance Characteristics
The growth-promoting capacities of McCoy’s 5A medium are tested in a medium containing 10% FBS using an appropriate cell line(s). Growth rates are examined through three subculture generations and compared with parallel cultures grown in standardized control medium. Cells are counted and growth is plotted as a logarithmic function of time in culture, and seeding efficiency, doubling time, and the final cell density are determined. During the testing period cultures are examined microscopically for a typical morphology and evidence of cytotoxicity.

Precautions
For In Vitro Use Only

Components	LM 005-01	LM 005-02
CaCl₂ (anhydrous)	100.00	100.00
KCl	400.00	400.00
MgSO₄(anhydrous)	98.00	98.00
NaCl	5100.00	5100.00
NaHCO₃	2200.00	2200.00
NaH₂PO₄·H₂O	580.00	580.00
Bacto-peptone 600	600.00	600.00
HEPES	-	5958.00
D-Glucose	3000.00	3000.00
Glutathione (reduced)	0.50	0.50
Phenol Red	10.00	10.00
L-Alanine	13.90	13.90
L-Arginine·HCl	42.10	42.10
L-Asparagine	45.00	45.00
L-Aspartic Acid	20.00	20.00
L-Cysteine	31.50	31.50
Glycine	7.50	7.50
L-Glutamic Acid	22.10	22.10
L-Glutamine	219.20	219.20
L-Histidine·HCl·H₂O	21.00	21.00
L-Hydroxyproline	19.70	19.70
L-Isoleucine	39.40	39.40
L-Leucine	39.40	39.40
L-Lysine·HCl	36.50	36.50
L-Methionine	15.00	15.00
L-Phenylalanine	16.50	16.50
L-Proline	17.30	17.30
L-Serine	26.30	26.30
L-Threonine	17.90	17.90
L-Tryptophan	3.10	3.10
L-Tyrosine·2Na·2H₂O	26.20	26.20
L-Valine	17.60	17.60
Asxorbic Acid	0.50	0.50
Biotin	0.20	0.20
Choline Chloride	5.00	5.00
D-Ca Panto thenate	0.20	0.20
Folic Acid	10.00	10.00
i-Inositol	36.00	36.00
Niacinamide	0.50	0.50
Nicotinic Acid	0.50	0.50
Para-aminobenzoic Acid	1.00	1.00
Pyridoxal·HCl	0.50	0.50
Pyridoxine·HCl	0.50	0.50
Riboflavin	0.20	0.20
Thiamine·HCl	0.20	0.20
Vitamin B₁₂	2.00	2.00

Product Profile	LM 005-01	LM 005-02
Appearance	Red transparent solution	Red transparent solution
pH at RT	7.0 ~ 7.6	7.0 ~ 7.6
Osmolality	272 ~ 300 mOsm/kg H₂O	315 ~ 349 mOsm/kg H₂O
Endotoxin	£ 1.0 EU/ml	£ 1.0 EU/ml
Sterility	Sterilized by 0.2 mm filtration system. Sterility tests are performed in accordance with protocols described in USP.

References

McCoy, T.A., Maxwell, M. and Kruse, P.F., (1959). Amino Acid Requirement of the Novioff Hepatoma In Vitro. Proc. Soc. Exp. Biol. Med. 100, 115-118.
Patterson, M.K. and Dell’orco, R.T., (1978). Preparation of McCoy’s Medium 5A. Tissue Culture Association Manual. 4, 737-740.
Hsu, T.C. and Kellogg, D.S., (1960). Primary Cultivation and Continuous Propagation In Vitro of Tissues from Small Biopsy Specimens. J.N.C.I. 25, 221-231.
Iwakata, S. and Grace, J.T., (1964). Cultivation in Vitro of Myeoblasts from Human Leukemia. New York State Journal of Med. 64, 2279-2282.
Morton, H.J., (1970). A Survey of Commercially Available Tissue Culture Media. In Vitro. 6, 89.