Shields and Sang M3 Insect Medium (1X), Liquid
With L-glutamine
With potassium bicarbonate
Insect cell culture tested

Catalog Number LM 504-01
Storage Temperature 2~8°C

Product Description
A variety of cell types developed when embryo cells of Drosophila melanogaster were cultured in Shields and Sang M3 Insect Medium. Nerve, muscles, fat-body, chitin-secreting, and macrophage-like cells (possibly haemocytes) appeared in this medium. And this medium has been found to support the rapid growth of both primary and established cultures of cells derived from Drosophila melanogaster. Drosophila cells have been employed to study a variety of biological processes including genetics, endocrinology, physiology and cell biology as well as recombinant protein expression.

LM 504-01 contains 600 mg/L L-glutamine and 500 mg/L potassium bicarbonate. The selection of a nutrient medium is strongly influenced by (1) type of cell, (2) type of culture (monolayer, suspension, or clonal), and (3) degree of chemical definition necessary. It is important to review the literature for recommendations concerning medium, supplementation and physiological parameters required for a specific cell line.

Storage/Stability
The liquid medium should be stored at 2~8°C in the dark. Deterioration of the liquid medium may be recognized by (1) precipitate or particulate matter throughout the solution, (2) cloudy appearance, (3) color change, and/or (4) pH change. The nature of supplements added may affect storage conditions and shelf life of the medium. Product label bears expiration date.

Biological Performance Characteristics
The growth-promoting capacities of Shields and Sang M3 Insect Medium are tested in a medium containing 5~20% FBS using an appropriate insect cell line(s). Growth rates are examined through three subculture generations and compared with parallel cultures grown in standardized control medium. Cells are counted and growth is plotted as a logarithmic function of time in culture, and seeding efficiency, doubling time, and the final cell density are determined. During the testing period cultures are examined microscopically for a typical morphology and evidence of cytotoxicity.

Precautions
For In Vitro Use Only

* In effort to cater to the individual needs of our clients, WelGENE provides custom products (classified as a “special order”) with variations made to the general specifications listed on the catalogue. Variations can be made in regards to 1. elimination of certain materials 2. alteration of chemical portions 3. addition of new materials and 4. change in pH.