L-Glutamine Solution (200mM)
Contains 29.2 mg/mL L-glutamine
in 0.85% NaCl solution
Sterile-filtered
Endotoxin tested
Cell culture tested

Catalog Number LS 002-01
Storage Temperature -5~-20°C

Product Description
L-Glutamine, one of amino acids, is necessary for the growth of cells. It serves as the precursor for other amino acids, such as glutamic acid, asparagine, tryptophan and histidine, through deamination. And it is the nitrogen source for several important biochemicals such as glucosamine-6-phosphate, NAD+, p-aminobenzoic acid, carbamyl phosphate, urea, arginine, CTP, AMP, GMP, and so on. Although being essential for in vivo, in vitro cell growth and function, L-glutamine in solution is spontaneously degraded to ammonia that is toxic to cells.

LS 002-01 contains 29.2 g of L-glutamine in 1 L of 0.85% NaCl solution. Appropriate volume of L-glutamine solution should be aseptically added to the medium. Concentrations widely used are as follows:

The selection of a nutrient medium is strongly influenced by (1) type of cell, (2) type of culture (monolayer, suspension, or clonal) and (3) degree of chemical definition necessary. It is important to review the literature for recommendations concerning medium, supplementation and physiological parameters required for a specific cell line.

Storage/Stability
L-Glutamine solution should be stored at -5~-20°C in the dark. Deterioration of the liquid may be recognized by (1) precipitate or particulate matter throughout the solution, (2) cloudy appearance, (3) color change, and/or (4) pH change. The nature of supplements added may affect storage conditions and shelf life of the medium. Product label bears expiration date.

Biological Performance Characteristics
The growth-promoting capacity of L-glutamine solution is tested in a medium containing 10% FBS using an appropriate cell line(s). Growth rates are examined through three subculture generations and compared with parallel cultures grown in standardized control medium. Cells are counted and

growth is plotted as a logarithmic function of time in culture, and seeding efficiencies, doubling time, and final cell densities are determined. During the testing period cultures are examined microscopically for a typical morphology and evidence of cytotoxicity.

Precautions

For In Vitro Use Only