MCDB 105 Medium
With L-glutamine
With 25 mM HEPES
Without sodium bicarbonate

Catalog Number LM 016-01
Storage Temperature 2~8°C

Product Description
MCDB Media were initially formulated to support low-protein or serum-free growth of specific cell types. Each MCDB medium is customized for optimally balanced nutritional environment that selectively promoted growth of specific cell. MCDB 105 medium is variations of the MCDB 104 medium, appropriate for long term survival of human diploid fibroblast-like cells (Wi-38, MRC-5, IMR-90).

LM 016-01 contains the 365.30 mg/L L-glutamine and 25 mM HEPES, but no sodium bicarbonate. The selection of a nutrient medium is strongly influenced by (1) type of cell, (2) type of culture (monolayer, suspension, clonal), and (3) degree of chemical definition necessary. It is important to review the literature for recommendations concerning medium, supplementation and physiological parameters required for a specific cell line.

Storage/Stability
The liquid medium should be stored at 2~8°C in the dark. Deterioration of the liquid medium may be recognized by (1) precipitate or particulate matter throughout the solution, (2) cloudy appearance, (3) color change, and/or (4) pH change. The nature of supplements added may affect storage conditions and shelf life of the medium. Product label bears expiration date.

Biological Performance Characteristics
The growth-promoting capacities of the liquid media are tested in a medium containing 10% FBS using an appropriate cell line(s). Growth rates are examined through three subculture generations and compared with parallel cultures grown in standardized control medium. Cells are counted and growth is plotted as a logarithmic function of time in culture, and seeding efficiency, doubling time, and final cell densities are determined. During the testing period cultures are examined microscopically for a typical morphology and evidence of cytotoxicity.

Precautions
For In Vitro Use Only

* In effort to cater to the individual needs of our clients, Welgene provides custom products (classified as a “special order”) with variations made to the general specifications listed on the catalogue. Variations can be made in regards to 1. elimination of certain materials 2. alteration of chemical portions 3. addition of new materials and 4. change in pH.

Comparison of liquid MCDB Media

Applications of some MCDB Media