M9 Minimal Medium, Liquid
Catalog Number MM 004-01
Storage Temperature 2~8°C

Product Description
M9 minimal medium is used to culture microbes such as E.coli and bacteriophge M13, in addition to amplifying plasmid. The composure of M9 minimal medium, which consists of the minimal nutritional requirements in supporting cell growth, is known in its entirety. Therefore the effect of certain ingredients on cell growth can be researched by adding supplements of the researcher’s choice.

Storage/Stability
M9 minimal medium should be stored at 2~8°C. Deterioration of M9 minimal medium may be recognized by (1) precipitate or particulate matter throughout the solution, (2) cloudy appearance, (3) color change, and/or (4) pH change. The nature of supplements added may affect storage conditions and shelf life of 5X Minimal Salts solution. Product label bears expiration date.

Biological Performance Characteristics
Use the following instructions for preparing complete M9 minimal medium as a starting point. Additional supplementation may be required depending on the nutritional needs of the specific microbe to be cultured. Inoculate and incubate cultures on a rotary shaker at 33°C to 37°C for 18~48 hours.

Precautions
For In Vitro Use Only