Terrific Broth (TB), Liquid
Catalog Number MM 012-01
Storage Temperature 2~8°C

Product Description
Terrific broth (TB) is appropriate for mass production of plasmid and cosmid DNA. Terrific Broth is an enriched medium, which helps the recombinant strains of E. coli maintain an extended growth phase.

Storage/Stability
Terrific broth should be stored at 2~8°C. Deterioration of Terrific broth may be recognized by (1) precipitate or particulate matter throughout the solution, (2) cloudy appearance, (3) color change, and/or (4) pH change. Product label bears expiration date.

Biological Performance Characteristics
Terrific broth medium contained tryptone, yeast extract and potassium phosphates.The large amount of tryptone and yeast extract in the medium allows the bacteria to produce a higher plasmid yield. The tryptone and yeast extracts also provide additional nutrients and growth factors required for enhanced growth of recombinant strains of E. coli. Glycerol is added as an additional carbohydrate source in this medium. The potassium phosphates are present to prevent a drop in pH of the medium during bacterial growth. This currently consists of tests for pH, sterility, and plasmid extraction efficiency using DH5α E. coli.

Precautions
For In Vitro Use Only