Phosphate Buffered Salines  (PBS), Liquid
Cell Culture Tested

Catalog Number LB 004-01 (1X), LB 204-01 (10X)
Catalog Number LB 004-02 (1X), LB 204-02 (10X)

Storage Temperature 15~30°C

Product Description
Buffered saline (BS) is one of the balanced salt solutions bearing the buffering capacity and maintaining the osmotic balance similar to the natural condition of body. Of many salines, phosphate-buffered saline (PBS) is widely used because the buffering capacity of phosphate is effective at in vivo neutral pH with major roles in maintaining viability or integrity rather than promoting growth or functions of cells or molecules. Other principal functions of BS are as follows; (1) dissolve or dilute materials for use in cell culture, (2) provides inorganic salts essential for the basic cell metabolism, (3) maintains the osmotic balance, (4) maintains pH with buffering capacity, and (5) eliminates certain components as serum or trypsin.

Storage/Stability
PBS should be stored at 15~30°C. For long time storage, store at 2~8°C. Deterioration of PBS may be recognized by (1) precipitate or particulate matter throughout the solution, (2) cloudy appearance, (3) color change, and/or (4) pH change. The nature of supplements added may affect storage conditions and shelf life of PBS. Product label bears expiration date.

Biological Performance Characteristics
The growth-maintaining capacity of PBS is tested in an appropriate medium using an appropriate cell line(s) as a washing solution of trypsin (or trypsin-EDTA). Growth rates are examined through three subculture generations and compared with parallel cultures grown in same medium treated by a standardized control washing solution. Cells are counted and growth is plotted as a logarithmic function of time in culture, and seeding efficiency, doubling time, and the final cell density are determined. During the testing period, cultures are examined microscopically for a typical morphology and evidence of cytotoxicity.

Precautions
For In Vitro Use Only

* In effort to cater to the individual needs of our clients, WelGENE provides custom products (classified as a “special order”) with variations made to the general specifications listed on the catalogue. Variations can be made in regards to 1. elimination of certain materials 2. alteration of chemical portions 3. addition of new materials and 4. change in pH.

Comparison of PBS