Phosphate Buffered Saline
(PBS) (10X), pH 7.4
Contains 1370 mM NaCl
27 mM KCl
100 mM Na₂HPO₄
20 mM KH₂PO₄
DNase, RNase and protease-none detected

Catalog Number ML 008-02
Storage Temperature 15~30°C

Product Description
Buffered saline (BS) is one of the balanced salt solutions containing the buffering capacity and maintaining osmotic balance similar to the natural condition of body. Of many salines, phosphate-buffered saline (PBS) is widely used because the buffering capacity of phosphate is effective at neutral pH like in vivo, and PBS’s major role is that maintaining viability or integrity than promoting growth or functions of cells or molecules. Other principal functions of BS are the followings; (1) dissolve or dilute materials for use in cell culture, (2) provides inorganic salts essential for the basic cell metabolism, (3) maintains the osmotic balance, (4) maintains pH with buffering capacity, and (5) eliminates certain components as serum or trypsin.

ML 008-02 contains 80.0 g of NaCl, 2.0 g of KCl, 14.4 g of Na2HPO4, and 2.4 g fo KH₂PO₄ in 1 L of ultra pure water (ML 019-02). Dilution of the concentrated PBS buffer produces a 1X PBS buffer with 137 mM NaCl, 2.7 mM KCl, 10 mM Na₂HPO₄, and 2 mM KH₂PO₄, pH approx. 7.4.

Storage/Stability
PBS should be stored at 15~30°C. For long time storage, store at 2~8°C.  Deterioration of the PBS may be recognized by (1) precipitate or particulate matter throughout the solution, (2) cloudy appearance, (3) color change, (4) pH change and/or (5) temperature change. The nature of supplements added may affect storage conditions and shelf life of the PBS. Product label bears expiration date.

Biological Performance Characteristics
The growth-maintaining capacities of PBS are tested in a medium containing 10% FBS using an appropriate call line(s) as a washing solution of trypsin (or trypsin-EDTA). Growth rates are examined through three subculture generations and compared with parallel cultures grown in standardized control medium. Cells are counted and growth is plotted as a logarithmic function of time in culture, and seeding efficiency, doubling time, and the final cell density are determined. During the testing period, cultures are examined microscopically for a typical morphology and evidence of cytotoxicity.

Precautions
For In Vitro Use Only