Water
Cell and Tissue Culture Grade
RO filtered
Endotoxin filtered
Cell culture tested

Catalog Number LS 016-01
Storage Temperature 15~30°C

Product Description
H2O, Water, Hydrogen oxide
FW 18.02
H 11.19%, O 88.81%
(Liq.) Temperature of maximal density; 3.98°C
d^3.98 1.000000 g/ml (0.999972 g/cc)
d₄²⁵  0.997 g/cc
d⁰ (ice) 0.917 g/cc
Melting point 0°C, Boiling point 100°C
One liter saturated vapor weighs 0.5974 g at 100°C at 760 mm
Critical Temperature 374.2°C, Critical Pressure 218 atm
Specific Heat (liq; 14°) 1.000 cal/g/°C
Latent Heat of Fusion 1.436 kcal/mole
Latent Heat of Vaporization 9.717 kcal/mole
Water is the most universal solvent and is also the most common component in natural cells and tissues.

LS 016-01 is the cell/tissue culture grade pure water prepared through reverse osmosis (RO) filtration, 2 steps of endotoxin removal filtration  and final ultra pure water system resulting in the water resistance of max. 18 MΩ. By these procedures, impurities are removed completely. Hence, the experimental errors due to lab water can be reduced and the high reproducibility can be provided. Cell/Tissue Culture Grade Pure Water may be used for dissolving powder media and reagents, and other various cell/tissue culture experiments.

Storage/Stability
Cell/Tissue Culture Grade Pure Water should be stored at 15~30°C. Deterioration of the solution may be recognized by (1) precipitate or particulate matter throughout the solution, (2) cloudy appearance, (3) color change, and/or (4) pH change. Product label bears expiration date.

Biological Performance Characteristics
The growth-promoting capacity of Cell/Tissue Culture Grade Pure Water is tested in a medium containing 10% FBS using an appropriate cell line(s). Growth rates are examined through three subculture generations and compared with parallel cultures grown in standardized control medium. Cells are counted and growth is plotted as a logarithmic function of time in culture, and seeding efficiency, doubling time, and the final cell density are determined. During the testing period cultures are examined microscopically for a typical morphology and evidence of cytotoxicity.

Precautions
For In Vitro Use Only