Waymouth MB 752/1 Medium, Liquid
Without L-glutamine

Catalog Number LM 015-01
Storage Temperature 2~8°C

Product Description
For cultivation of mouse L929 cells in a serum-free environment, Waymouth Medium MB 752/1 was developed as a totally defined synthetic medium. In this medium, L929 cells were cultivated for more than 30 passages and demonstrated a 24-hr doubling time. An Waymouth medium may be used for organ culture and establishment of carcinoma cell lines from pleural effusion.

LM 015-01 contains the high D-glucose concentration of 5000 mg/L, 350 mg/L L-glutamine, and 2240 mg/L sodium bicarbonate. The selection of a nutrient medium is strongly influenced by (1) type of cell, (2) type of culture (monolayer, suspension, or clonal), and (3) degree of chemical definition necessary. It is important to review the literature for recommendations concerning medium, supplementation and physiological parameters required for a specific cell line.

Storage/Stability
The liquid medium should be stored at 2~8°C in the dark. Deterioration of the liquid medium may be recognized by (1) precipitate or particulate matter throughout the solution, (2) cloudy appearance, (3) color change, and/or (4) pH change. The nature of supplements added may affect storage conditions and shelf life of the medium. Product label bears expiration date.

Biological Performance Characteristics
The growth-promoting capacities of Waymouth medium are tested in a 1X medium using an appropriate cell line(s). Growth rates are examined through three subculture generations and compared with parallel cultures grown in standardized control medium. Cells are counted and growth is plotted as a logarithmic function of time in culture, and seeding efficiency, doubling time, and the final cell density are determined. During the testing period cultures are examined microscopically for a typical morphology and evidence of cytotoxicity.

Precautions
For In Vitro Use Only