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Cell Culture Media
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Classical
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GenGro medium
DMEM Dulbecco’s Modified Eagle’s Medium
DMEM / F-12 1:1 Mixture
L-15 Leibovitz’s L-15 Medium
IMDM Iscove’s Modified Dulbecco’s Medium
McCoy’s 5A Medium
M199 Meidum 199
MEM Minimum Essential Medium Eagle
α-MEM Minimum Essential Medium Eagle, Alpha
Ham's F-10 Nutrient Mixture
Ham's F-12 Nutrient Mixture
RPMI 1640 Medium
BME Basal Media Eagle
CMRL1066 Medium
Fischer’s Medium
Waymouth MB752/1 Medium
MCDB Medium
Williams’ Medium E
DMEM / F-12 3:1 Mixture
DMEM 2X Dulbecco’s Modified Eagle’s Medium 2X
M199 2X Meidum 199 2X
MEM 2X Minimum Essential Medium Eagle 2X
RPMI1640 Medium 2X
Serum Free and Protein free
Insect
Microbial
Culture-related
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Freezing Solutions
Antibiotics
Cell Dissociation Solution
Serum
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FBS
BCS
Fetalgro EX
Stem Cell
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Culture Media
Growth factor
Cell & Molecular Biology
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Buffers & Solutions
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DPBS (Dulbecco's Phosphate Buffered Saline)
EBSS (Earle’s Balanced Salt Solution)
HBSS (Hanks’ Balanced Salt Solution)
PBS (Phosphate-Buffered Saline)
Poly-D-Lysine (NEW)
HEPES Buffer
Sodium bicarbonate Solution
D-Glucose Solution
L-Glutamine Solution
Hydrochloric Acid Solution
MEM Amino Acid Solution
MEM Non-essential Amino Acid Solution
MEM Vitamin Solution
Phenol Red Solution
RPMI 1640 Amino Acid Solution
RPMI 1640 Vitamin Solution
Sodium Chloride Solution
Sodium Hydroxide Solution
Sodium Pyruvate Solution
Trypan Blue Solution
Calcium Chloride Solution
Magnesium Sulfate Solution
Albumin, Bovine Fraction V Solution
Gelatin Solution
ITSP, Insulin-Transferrin-Selenium Pyruvate Supplement
Soy Protein Solution
Peptone Solution
Sodium Butyrate Solution
Sodium Propionate Solution
Insulin Solution
Grace’s Amino Acid Solution
Grace’s Vitamin Solution
Poloxamer 188
Yeast Extract Solution
L-Alanyl-L-Glutamine Solution
Waters
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SuperPure Water
Water
Water, Cell and Tissue Culture Grade
Transfection
Nucleic acid
Protein
Liquid Reagents
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Acrylamide Solution
Acrylamide/Bis-Acrylamide Solution
Antibiotic Slutions
EDTA Solution
HCl Solution
PBS Solution
SDS Solution
Sodium Acetate Solution
NaCl Solution
SSC Buffer Solution
SSPE Buffer Solution
TE Buffer Solution
TAE Buffer Solution
TBE Buffer Solution
Tris-HCl Buffer Solution
X-Gal Solution
Sodium Phosphate Buffer Solution
Alkaline Lysis Soluton
NaOH Solution
TBS Solution
Stacking Gel Buffer
Running Buffer (192mM Glycine)
Running Buffer (250mM Glycine)
Sample Loading Buffer
Gel Staining Solution
Gel Destaining Solution
Transfer Buffer
TBS Solution (for blocking)
Washing Buffer
Membrane staining solution
Membrane destaining solution
Glycine Buffer
Borate Buffer
MES Buffer
Plastic Ware
Package Savings
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Bulk Products
FBS Package
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FBS PREMIUM PKG
FBS CERTIFIED GRADE PKG
FBS PREMIUM HEAT PKG
FBS CERTIFIED GRADE HEAT PKG
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Cell Culture Media
Classical
GenGro medium
DMEM Dulbecco’s Modified Eagle’s Medium
DMEM / F-12 1:1 Mixture
L-15 Leibovitz’s L-15 Medium
IMDM Iscove’s Modified Dulbecco’s Medium
McCoy’s 5A Medium
M199 Meidum 199
MEM Minimum Essential Medium Eagle
α-MEM Minimum Essential Medium Eagle, Alpha
Ham's F-10 Nutrient Mixture
Ham's F-12 Nutrient Mixture
RPMI 1640 Medium
BME Basal Media Eagle
CMRL1066 Medium
Fischer’s Medium
Waymouth MB752/1 Medium
MCDB Medium
Williams’ Medium E
DMEM / F-12 3:1 Mixture
DMEM 2X Dulbecco’s Modified Eagle’s Medium 2X
M199 2X Meidum 199 2X
MEM 2X Minimum Essential Medium Eagle 2X
RPMI1640 Medium 2X
Serum Free and Protein free
Insect
Microbial
Culture-related
Freezing Solutions
Antibiotics
Cell Dissociation Solution
Serum
FBS
BCS
Fetalgro EX
Stem Cell
Culture Media
Growth factor
Cell & Molecular Biology
Buffers & Solutions
DPBS (Dulbecco's Phosphate Buffered Saline)
EBSS (Earle’s Balanced Salt Solution)
HBSS (Hanks’ Balanced Salt Solution)
PBS (Phosphate-Buffered Saline)
Poly-D-Lysine (NEW)
HEPES Buffer
Sodium bicarbonate Solution
D-Glucose Solution
L-Glutamine Solution
Hydrochloric Acid Solution
MEM Amino Acid Solution
MEM Non-essential Amino Acid Solution
MEM Vitamin Solution
Phenol Red Solution
RPMI 1640 Amino Acid Solution
RPMI 1640 Vitamin Solution
Sodium Chloride Solution
Sodium Hydroxide Solution
Sodium Pyruvate Solution
Trypan Blue Solution
Calcium Chloride Solution
Magnesium Sulfate Solution
Albumin, Bovine Fraction V Solution
Gelatin Solution
ITSP, Insulin-Transferrin-Selenium Pyruvate Supplement
Soy Protein Solution
Peptone Solution
Sodium Butyrate Solution
Sodium Propionate Solution
Insulin Solution
Grace’s Amino Acid Solution
Grace’s Vitamin Solution
Poloxamer 188
Yeast Extract Solution
L-Alanyl-L-Glutamine Solution
Waters
SuperPure Water
Water
Water, Cell and Tissue Culture Grade
Transfection
Nucleic acid
Protein
Liquid Reagents
Acrylamide Solution
Acrylamide/Bis-Acrylamide Solution
Antibiotic Slutions
EDTA Solution
HCl Solution
PBS Solution
SDS Solution
Sodium Acetate Solution
NaCl Solution
SSC Buffer Solution
SSPE Buffer Solution
TE Buffer Solution
TAE Buffer Solution
TBE Buffer Solution
Tris-HCl Buffer Solution
X-Gal Solution
Sodium Phosphate Buffer Solution
Alkaline Lysis Soluton
NaOH Solution
TBS Solution
Stacking Gel Buffer
Running Buffer (192mM Glycine)
Running Buffer (250mM Glycine)
Sample Loading Buffer
Gel Staining Solution
Gel Destaining Solution
Transfer Buffer
TBS Solution (for blocking)
Washing Buffer
Membrane staining solution
Membrane destaining solution
Glycine Buffer
Borate Buffer
MES Buffer
Plastic Ware
Package Savings
Bulk Products
FBS Package
FBS PREMIUM PKG
FBS CERTIFIED GRADE PKG
FBS PREMIUM HEAT PKG
FBS CERTIFIED GRADE HEAT PKG
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Cell & Tissue Culture
Mammalian and Insect cell culture
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세포배양의 역사
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세포배양시 필요한 장치
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생세포수의 계산
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살균용 ethanol의 농도
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부착 세포 배양과 부유 세포 배양
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세포의 동결 보존
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동결 보존된 세포의 배양
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세포의 계대배양
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세포주(continuous cell lines)
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곤충 세포의 배양
Classical Media
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Classical Media 종류별 성분 비교
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세포 배양 배지의 제조에 사용하는 초순수물
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Classical Media 성분
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Hanks' salt와 Earle's salt의 차이점
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세포 배양 배지에 적용되는 완충 작용
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L-Glutamine의 함량이 다른 아미노산보다 높은 이유?
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L-Alanyl-L-Glutamine과 L-Glutamine
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무균 시험(steriliy test)
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내독소 시험(endotoxin test)
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Classical Media 종류별 성분 비교
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세포 배양 배지의 제조에 사용하는 초순수물
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Classical Media 성분
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Hanks' salt와 Earle's salt의 차이점
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세포 배양 배지에 적용되는 완충 작용
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L-Glutamine의 함량이 다른 아미노산보다 높은 이유?
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L-Alanyl-L-Glutamine과 L-Glutamine
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무균 시험(steriliy test)
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내독소 시험(endotoxin test)
Serum-Free and Protein Free Media
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Serum-free media와 protein-free media
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Serum-free media와 protein-free media의 장점
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Serum-free media와 protein-free media의 단점
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Serum-free media 또는 protein-free media를 PBMC의 배양
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Serum-free hybridoma media 또는 protein-free hybridoma media로의 적응 방법
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Serum-Free CHO media 또는 protein-free CHO media로의 적응 방법
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혈청 대체 물질 (serum replacements)
Supplements
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세포 배양에서 혈청의 역할
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혈청의 종류
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혈청의 성분
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혈청의 열불활성화 (heat-inactivation)
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혈청의 저장
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혈청의 해동 방법
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세포 배양시 사용하는 항생제
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Geneticin(G418 sulfate)
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호르몬(hormone)
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Soybean trypsin inhibitor
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성장 인자(growth factors)
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