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Molecular Biology

Technical Tables for Molecular Biology

▶ Acrylamide Gel Electrophoresis of Xylene Cyanole FF and Bromophenol Blue

▶Acrylamide Gel Electrophoresis of Proteins

▶ Agarose Gel Electrophoresis of Xylene Cyanole FF and Bromophenol Blue

▶ Amino Acids

▶ Antibiotic Solutions

▶ Genetic Markers

▶ Nucleic Acid Data

▶ pH of 50 mM Tris-HCl Buffer Solutions

▶ Physical Constants of the Nucleoside Triphosphates

▶ Recommended Gel Percentages for Separation of Linear DNA

▶ Specifications of Common Vectors

Enzymes for DNA and RNA Modification

▶ DNA Polymerases

▶ Phosphatases

▶ Reverse Transcriptases

▶ RNA Polymerases

▶ Others

Protocols

▶ DNA Mini-Agarose Gel Electrophoresis

▶ Transformation of E. coli (CaCl2 and heat-shock method)

▶ Mini-Prep of Plasmid DNA (Alkaline Lysis Method)

Preparation of Liquid solutions and Buffers

▶ 45% Acrylamide Solution

▶ Actinomycin D Solution

▶ 0.1 M Adenosine 5'-Triphosphate (ATP) Solution

▶ 10 M Ammonium Acetate Solution

▶ 10% Ammonium Persulfate (APS) Solution

▶ 20 mg/ml 5-Bromo-4-Chloro-3-Indolyl-β-D-Galactopyranoside (X-Gal) Solution

▶ 50 mg/ml 5-Bromo-4-Chloro-3-Indolyl Phosphate (BCIP) Solution

▶ 1 M CaCl2 Solution

▶ 50 mM Deoxyribonucleoside Triphosphate (dNTP) Solutions (Each)

▶ 1 M Dithiothreitol (DTT) Solution

▶ 10 mg/ml Ethidium Bromide (EtBr) Solution

▶ 0.5 M Ethylenediaminetetraacetic Acid (EDTA) Solution, pH 8.0

▶ 200 mg/ml Isopropylthio-β-D-Galactoside (IPTG) Solution

▶ 50 mg/ml Nitro Blue Tetrazolium (NBT) Solution

▶ Phenol Equilibration

▶ Phosphate-Buffered Saline (PBS) Solution

▶ Potassium Acetate Solution(For Alkaline Lysis Method of Plasmid Preparation)

▶ 20X Saline-Sodium Citrate (SSC) Buffer

▶ 20X Saline-Sodium Phosphate-EDTA (SSPE) Buffer

▶ 3 M Sodium Acetate (pH 5.2 and pH 7.0) Solution

▶ 20% Sodium Dodecyl Sulfate (SDS, Lauryl Sulfate Sodium Salt) Solution