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Ethylenediaminetetraacetic Acid (EDTA) Solutions, pH 8.0
DNase, RNase and protease-none detected
Catalog Number ML 005-01 (0.5 M)
ML 005-02 (1.0 M)
Storage Temperature 15~30°C
Ethylenediaminetetraacetic acid (EDTA) combines with cations such as Mg2+ or Ca2+ to from a complex compound. EDTA has 4 negative charges for each molecule, and reacts with the diatomic positive charges of two cations. These cations generally assist enzymes (DNase) in hydrolyzing DNA. By adding EDTA to solutions that contain nucleic acid (TE, TAE, TBE, TPE buffer etc.) potential DNase activity can be deterred. In molecular biology studies, cations are commonly used to deter enzyme reactions by preventing the activation of assistance factors such as complementary enzymes. Cations also reinforce the cell wall of E. coli and many other microbes, and can be removed by adding EDTA.
ML 005−01 contains 186.1 g/L EDTA·2Na·2H2O and 20 g/L NaOH in Ultra Pure Water (ML019-02).
ML 005−02 contains 372.2 g/L EDTA·2Na·2H2O and 40 g/L NaOH in Ultra Pure Water (ML019-02).
The concentrated EDTA solutions should be stored at 15~30°C. Deterioration of the solution may be recognized by (1) precipitate or particulate matter throughout the solution, (2) cloudy appearance, (3) color change, and/or (4) pH change. Product label bears expiration date.
For In Vitro Use Only