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10X Tris-Borate-EDTA Buffer
(10X TBE Buffer)
DNase, RNase and protease-none detected
Catalog Number ML 016-01
Storage Temperature 15~30°C
TBE buffer is used for qualification and quantification of non-denaturing double stranded DNA through agarose gel electrophoresis. Agarose melts in TBE buffer and DNA is running in agarose gel placed in TBE buffer. The electrophoretic mobility of DNA is affected by the composition and ionic strength of the electrophoresis buffer. Tris-acetate-EDTA (TAE), tris-borate-EDTA (TBE), and tris-phosphate-EDTA (TPE) are available for electrophoresis of native double-stranded DNA. TBE buffer has significantly higher buffering capacity than that of TAE buffer. TBE is used at a working strength of 1X for polyacrylamide gel electrophoresis, twice the strength usually used for agarose gel electrophoresis. The buffer reservoirs of the vertical tanks used for polyacrylamide gel electrophoresis are fairly small, and the amount of electric current passed through them is often considerable. 1X TBE is required to provide adequate buffering power. Applied voltages of less than 5 V/cm (distance between the electrodes of the unit) are recommended for maximum resolution.
ML 016-01 contains 109 g of tris base, 55.65 g of boric acid, and 7.44 g of EDTA·2Na·2H2O in 1 L of ultra pure water (ML 019-02). Dilutions of 10X TBE buffer to 0.5X buffer (45 mM tris-borate, pH approx. 8.3, and 1 mM EDTA) and to 1X buffer (90 mM tris-borate, pH approx. 8.3, and 2 mM EDTA) should be used in agarose gel and polyacrylamide gel electrophoresis, respectively.
The concentrated TBE buffers should be stored at 15~30°C. Deterioration of the solution may be recognized by (1) precipitate or particulate matter throughout the solution, (2) cloudy appearance, (3) color change, and/or (4) pH change. Product label bears expiration date.
Biological Performance Characteristics
The biological characteristics of the concentrated TBE buffers are tested using polyacrylamide gel electrophoresis of DNA in 1X working solution, and compared with the resolution of the parallel DNA bands in standardized control solution.
For In Vitro Use Only