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Tris-Acetate-EDTA Buffer Solutions
DNase, RNase and protease-none detected
Catalog Number ML 015-01 (10X)
ML 015-02 (50X)
Storage Temperature 15~30°C
TAE buffer is used for qualification and quantification of DNA and non-denaturing RNA through agarose gel electrophoresis. Agarose melts in TAE buffer and DNA is running in agarose gel placed in TAE buffer. The electrophoretic mobility of DNA is affected by the composition and ionic strength of the electrophoresis buffer. Tris-acetate-EDTA (TAE), tris-borate-EDTA (TBE), and tris-phosphate-EDTA (TPE) are available for electrophoresis of native double- stranded DNA. In TAE buffer, the resolution of supercoiled DNAs is better than in TBE. For historical reasons, TAE is the most commonly used buffer with the advantage of the lower cost than TBE or TPE. Applied voltages of less than 5 V/cm (distance between the electrodes of the unit) are recommended for maximum resolution.
ML 015−01 contains 48.44 g of tris base, 11.42 mL of glacial acetic acid, and 37.22 g of EDTA·2Na·2H2O in 1 L of ultra pure water (ML 019-02).
ML 015−02 contains 242.2 g of tris base, 57.1 mL of glacial acetic acid, and 186.1 g of EDTA·2Na·2H2O in 1 L of ultra pure water (ML 019-02). Dilution of the concentrated TAE buffer produces a 1X TAE buffer with 40 mM tris-acetate and 1 mM EDTA, pH approx. 8.3, or a 0.5X TAE buffer with 20 mM tris-acetate and 0.5 mM EDTA, pH approx. 8.3.
The concentrated TAE buffers should be stored at 15~30°C. Deterioration of the solution may be recognized by (1) precipitate or particulate matter throughout the solution, (2) cloudy appearance, (3) color change, and/or (4) pH change. Product label bears expiration date.
Biological Performance Characteristics
The biological characteristics of the concentrated TAE buffers are tested using agarose gel electrophoresis of DNA in 1X working solution, and compared with the resolution of the parallel DNA bands in standardized control solution.
For In Vitro Use Only