Cart0
50X TAE (ML015-02)

50X TAE (ML015-02)


  오후 1시 이전 주문은 당일 발송됩니다.
  냉장, 냉동 제품은 금요일에는 발송이 불가능하며, 차주 월요일에 발송됩니다.
  재고 소진 시 배송이 지연 될 수 있습니다.(제품마다 생산소요기간 상이)
Size
Price
Quantity
100ML
₩14,000
1L
₩84,000
 
Special Order for bulk

Contact Us
Product Details

제품 설명서 (PDF 파일 다운로드) MSDS (PDF 파일 다운로드)

 

Tris-Acetate-EDTA Buffer Solutions
(TAE Buffers)
DNase, RNase and protease-none detected

Catalog Number ML 015-01 (10X)
                            ML 015-02 (50X)

Storage Temperature 15~30°C

Product Description
TAE buffer is used for qualification and quantification of DNA and non-denaturing RNA through agarose gel electrophoresis. Agarose melts in TAE buffer and DNA is running in agarose gel placed in TAE buffer. The electrophoretic mobility of DNA is affected by the composition and ionic strength of the electrophoresis buffer. Tris-acetate-EDTA (TAE), tris-borate-EDTA (TBE), and tris-phosphate-EDTA (TPE) are available for electrophoresis of native double- stranded DNA. In TAE buffer, the resolution of supercoiled DNAs is better than in TBE. For historical reasons, TAE is the most commonly used buffer with the advantage of the lower cost than TBE or TPE. Applied voltages of less than 5 V/cm (distance between the electrodes of the unit) are recommended for maximum resolution.

ML 01501 contains 48.44 g of tris base, 11.42 mL of glacial acetic acid, and 37.22 g of EDTA·2Na·2H2O in 1 L of ultra pure water (ML 019-02).

ML 01502 contains 242.2 g of tris base, 57.1 mL of glacial acetic acid, and 186.1 g of EDTA·2Na·2H2O in 1 L of ultra pure water (ML 019-02). Dilution of the concentrated TAE buffer produces a 1X TAE buffer with 40 mM tris-acetate and 1 mM EDTA, pH approx. 8.3, or a 0.5X TAE buffer with 20 mM tris-acetate and 0.5 mM EDTA, pH approx. 8.3.

Storage/Stability
The concentrated TAE buffers should be stored at 15~30°C. Deterioration of the solution may be recognized by (1) precipitate or particulate matter throughout the solution, (2) cloudy appearance, (3) color change, and/or (4) pH change. Product label bears expiration date.

Biological Performance Characteristics
The biological characteristics of the concentrated TAE buffers are tested using agarose gel electrophoresis of DNA in 1X working solution, and compared with the resolution of the parallel DNA bands in standardized control solution.

Precautions
For In Vitro Use Only