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Alkalin Lysis Solution III (ML021-03)

Alkalin Lysis Solution III (ML021-03)


  오후 1시 이전 주문은 당일 발송됩니다.
  냉장, 냉동 제품은 금요일에는 발송이 불가능하며, 차주 월요일에 발송됩니다.
  재고 소진 시 배송이 지연 될 수 있습니다.(제품마다 생산소요기간 상이)
Size
Price
Quantity
100ML
₩34,600
500ML
₩109,000
 
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Product Details

제품 설명서 (PDF 파일 다운로드) MSDS (PDF 파일 다운로드)

 

Alkaline lysis solution I, II, and III are solutions that extract small quantities of plasmid DNA from E. coli. 

Solution I destroys cell walls and is more effective when used together with lysozyme. 
Solution II breaks down cell membrane and deactivates protein contained in cells, basifying the cell suspension solution and separating one strand chromosomal cell DNA during the process. 
Solution III neutralizes basic cell suspension solution while precipitating single strand chromosomal DNA with large quantities of potassium.
The suspension solution is removed of protein through phenol extraction, after which plasmid DNA is enriched and purified through alcohol precipitation. 

 

Product Name Description Cat. No. Recommended Storage Temp. Size
Alkaline Lysis Solution I Components
50 mM Glucose
25 mM Tris-Cl (pH 8.0)
10 mM EDTA (pH 8.0)
Sterile-filtered
DNase, RNase and protease-none detected
Store at 2-8°C for long storage
ML 021-01 Store at 15-30°C 100ML, 500ML
Alkaline Lysis Solution II Components
0.2 N NaOH
1% (w/v) SDS
Sterile-filtered
DNase, RNase and protease-none detected
Before use, warm up to 37°C for 20 min
After use, close the cap tightly for preventing oxidation
ML 021-02 Store at 15-30°C 100ML, 500ML
Alkaline Lysis Solution III Components
3 M Potassium
5 M Acetate
pH 5.5
Sterile-filtered
DNase, RNase and protease-none detected
ML 021-03 Store at 15-30°C 100ML, 500ML