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Contains 10,000 units/mL of penicillin
10 mg/mL streptomycin sulfate
25 mg/mL amphotericin B
in 0.85% sodium chloride
For gram-positive, gram-negative bacteria, yeasts, and molds
Catalog Number LS 203-01
Storage Temperature -5~-20°C
Microbial contamination results in the lethal errors in cell and tissue culture. Animal cell divides into two daughter cells in several hours to days, however microbes such as bacteria, fungi, or yeasts are doubled in decades of minute to hours. Hence, overgrowing of these microbes inhibits the growth, proliferation, and function of animal cells. Microbial components or products can result the toxic effect to animal cells. Generally, in culture medium, antibiotics are added for inhibition of microbial growth. Though there are many types of antibiotics used in cell culture media, penicillin G, streptomicin, and ampho- tericin B are widely used.
LS 203-01 may be used as the antibiotic and antimycotic solution for preventing the contamination of bacteria, yeasts, and molds. The generally used concentrations of these antibiotic and antimycotic materials are 100,000 units/L for penicillin, 100 mg/L for streptomicin, and 0.25 mg/L for amphotericin B. Each concentration of antibiotic and antimycotic materials can be obtained by 1/100 dilution of this solution in medium.
* G (+), Gram-positive bacteria
* G (+, -), Gram-positive and Gram-negative bacteria
* Y, Yeasts
* M, Molds
Antibiotic antimycotic solution should be stored at -5~-20°C in the dark and can be stable for 3 days during cultivation of cells at 37°C. After 3 days, medium should be refreshed to a new medium. Deterioration of the liquid may be recognized by (1) precipitate or particulate matter throughout the solution, (2) cloudy appearance, (3) color change, and/or (4) pH change. The nature of supplements added may affect storage conditions and shelf life of the medium. Product label bears expiration date.