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Dulbecco's Modified Eagle's Medium (DMEM) (2X), Liquid
With sodium bicarbonate
Catalog Number LM 201-50 (Equal to LM001-07 at 1X)
Catalog Number LM 201-51 (Equal to LM001-10 at 1X)
Storage Temperature 2~8°C
For animal cell culture, Eagle’s Medium have been developed and modified since the original formulation appeared in the literature. Dulbecco’s Modified Eagle’s Medium (DMEM) is a modification of Minimum Essential Medium (MEM) that contains a higher concentration of amino acids and vitamins, as well as additional supplementary components, such as D-glucose (low or high, 1000 mg/L or 4500 mg/L, respectively).
LM 201-50 contains 1168 mg/L of L-glutamine and 7400 mg/L of sodium bicarbonate. LM 201-51 contains 1168 mg/L of L-glutamine, 7400 mg/L of sodium bicarbonate, 11916 mg/L of HEPES. But no phenol red. The selection of a nutrient medium is strongly influenced by (1) type of cell, (2) type of culture (monolayer, suspension, or clonal) and (3) degree of chemical definition necessary. It is important to review the literature for recommend- ations concerning medium, supplementation and physiological parameters required for a specific cell line.
The liquid medium should be stored at 2~8°C in the dark. Deterioration of the liquid medium may be recognized by (1) precipitate or particulate matter throughout the solution, (2) cloudy appearance, (3) color change, and/or (4) pH change. The nature of supplements added may affect storage conditions and shelf life of the medium. Product label bears expiration date.
Biological Performance Characteristics
The growth-promoting capacities of DMEM are tested in a medium containing 10% FBS using an appropriate cell line(s). Growth rates are examined through three subculture generations and compared with parallel cultures grown in standardized control medium. Cells are counted and growth is plotted as a logarithmic function of time in culture, and seeding efficiency, doubling time, and the final cell density are determined. During the testing period cultures are examined microscopically for a typical morphology and evidence of cytotoxicity. Test results are available upon request.
Preparation Procedure (1 L of 1X medium)
- Mix 500 ml of 2X Dulbecco's Modified Eagle's Medium and 400 ml of sterile cell/tissue culture grade water (LS 016-01) in an appropriate vessel.
- If the desired pH is different from the pH in table below, adjust pH using sterile 1 N HCl (LS 003-02) or 1 N NaOH (LS 012-02).
- Fill up to 1 L by adding sterile cell/tissue culture grade water.
For In Vitro Use Only