DMEM (LM001-04)
냉장, 냉동 제품은 금요일에는 발송이 불가능하며, 차주 월요일에 발송됩니다.
재고 소진 시 배송이 지연 될 수 있습니다.(제품마다 생산소요기간 상이)
| 제품 설명서 (PDF 파일 다운로드) | MSDS (PDF 파일 다운로드) | |
Dulbecco's Modified Eagle's Medium (DMEM) (1X), Liquid (Low Glucose)
With 1000 mg/L D-glucose
Without L-Glutamine
Without phenol red
Catalog Number LM 001-04
Storage Temperature 2~8°C
Product Description
For animal cell culture, Eagle’s Medium have been developed and modified since the original formulation appeared in the literature. Dulbecco’s Modified Eagle’s Medium (DMEM) is a modification of Minimum Essential Medium (MEM) that contains a higher concentration of amino acids and vitamins, as well as additional supplementary components, such as D-glucose (low or high, 1000 mg/L or 4500 mg/L, respectively).
LM 001-04 contains the low D-glucose concentration of 1000 mg/L, but no L-glutamine and phenol red. Add aseptically 20 mL of sterile 200 mM L-glutamine solution (LS 002-01) per 1 L of medium before use. The selection of a nutrient medium is strongly influenced by (1) type of cell, (2) type of culture (monolayer, suspension, clonal), and (3) degree of chemical definition necessary. It is important to review the literature for recommendations concerning medium, supplementation and physiological parameters required for a specific cell line.
Storage/Stability
The liquid medium should be stored at 2~8°C in the dark. Deterioration of the liquid medium may be recognized by (1) precipitate or particulate matter throughout the solution, (2) cloudy appearance, (3) color change, and/or (4) pH change. The nature of supplements added may affect storage conditions and shelf life of the medium. Product label bears expiration date.
Biological Performance Characteristics
The growth-promoting capacities of the liquid media are tested in a medium containing 10% FBS using an appropriate cell line(s). Growth rates are examined through three subculture generations and compared with parallel cultures grown in standardized control medium. Cells are counted and growth is plotted as a logarithmic function of time in culture, and seeding efficiency, doubling time, and final cell densities are determined. During the testing period cultures are examined microscopically for a typical morphology and evidence of cytotoxicity.
Precautions
For In Vitro Use Only
| Product Name | Description | Cat. No. | Recommended Storage Temp. | Size |
|---|---|---|---|---|
| Dulbecco’s Modified Eagle’s Medium (DMEM) (1X), liquid (high glucose) |
With 4500 mg/L D-glucose Without L-glutamine |
LM 001-01 | Store at 2-8°C | 500ML, 1L |
| Dulbecco’s Modified Eagle’s Medium (DMEM) (1X), liquid (low glucose) |
With 1000 mg/L D-glucose Without L-glutamine |
LM 001-02 | Store at 2-8°C | 500ML |
| Dulbecco’s Modified Eagle’s Medium (DMEM) (1X), liquid (high glucose) |
With 4500 mg/L D-glucose With 25 mM HEPES Without L-glutamine |
LM 001-03 | Store at 2-8°C | 500ML, 1L |
| Dulbecco’s Modified Eagle’s Medium (DMEM) (1X), liquid (low glucose) |
With 1000 mg/L D-glucose Without L-glutamine Without phenol red |
LM 001-04 | Store at 2-8°C | 500ML |
| Dulbecco’s Modified Eagle’s Medium (DMEM) (1X), liquid (high glucose) |
With 4500 mg/L D-glucose With L-glutamine With 110 mg/L sodium pyruvate |
LM 001-05 | Store at 2-8°C | 500ML, 1L |
| Dulbecco’s Modified Eagle’s Medium (DMEM) (1X), liquid (low glucose) |
With 1000 mg/L D-glucose With L-glutamine |
LM 001-06 | Store at 2-8°C | 500ML, 1L |
| Dulbecco’s Modified Eagle’s Medium (DMEM) (1X), liquid (high glucose) |
With 4500 mg/L D-glucose With L-glutamine |
LM 001-07 | Store at 2-8°C | 500ML, 1L |
| Dulbecco’s Modified Eagle’s Medium (DMEM) (1X), liquid (high glucose) |
With 4500 mg/L D-glucose With 110 mg/L sodium pyruvate Without L-glutamine |
LM 001-08 | Store at 2-8°C | 500ML, 1L |
| Dulbecco’s Modified Eagle’s Medium (DMEM) (1X), liquid (high glucose) |
With 4500 mg/L D-glucose With L-glutamine With 25 mM HEPES |
LM 001-09 | Store at 2-8°C | 500ML, 1L |
| Dulbecco’s Modified Eagle’s Medium (DMEM) (1X), liquid (high glucose) |
With 4500 mg/L D-glucose With L-glutamine With 25 mM HEPES Without phenol red |
LM 001-10 | Store at 2-8°C | 500ML |
| Dulbecco’s Modified Eagle’s Medium (DMEM) (1X), liquid (low glucose) |
With 1000 mg/L D-glucose With L-glutamine With 110 mg/L sodium pyruvate |
LM 001-11 | Store at 2-8°C | 500ML, 1L |
| Dulbecco’s Modified Eagle’s Medium (DMEM) (1X), liquid (low glucose) |
With 1000 mg/L D-glucose With L-glutamine With 25 mM HEPES With 110 mg/L sodium pyruvate |
LM 001-12 | Store at 2-8°C | 500ML, 1L |
| Dulbecco’s Modified Eagle’s Medium (DMEM) (1X), liquid (low glucose) |
With 1000 mg/L D-glucose With 25 mM HEPES With 110 mg/L sodium pyruvate Without L-glutamine |
LM 001-13 | Store at 2-8°C | 500ML, 1L |
| Dulbecco’s Modified Eagle’s Medium (DMEM) (1X), liquid (low glucose) |
With 1000 mg/L D-glucose With 110 mg/L sodium pyruvate Without L-glutamine |
LM 001-14 | Store at 2-8°C | 500ML, 1L |
| Dulbecco’s Modified Eagle’s Medium (DMEM) (1X), liquid (low glucose) |
With 1000 mg/L D-glucose With 110 mg/L sodium pyruvate Without L-glutamine Without phenol red |
LM 001-15 | Store at 2-8°C | 500ML, 1L |
* In effort to cater to the individual needs of our clients, Welgene provides custom products (classified as a “special order”) with variations made to the general specifications listed on the catalogue. Variations can be made in regards to 1. elimination of certain materials 2. alteration of chemical portions 3. addition of new materials and 4. change in pH.
Comparison of liquid DMEM
| Cat. No. | D-Glucose (mg/L) | L-Glutamine (mg/L) | HEPES (mg/L) | Sodium Pyruvate (mg/L) | Phenol Red (mg/L) | Sodium Bicarbonate (mg/L) |
|---|---|---|---|---|---|---|
| LM 001-01 | 4500.00 | - | - | - | 15.00 | 3700.00 |
| LM 001-02 | 1000.00 | - | - | - | 15.00 | 3700.00 |
| LM 001-03 | 4500.00 | - | 5958.00 (25 mM) | - | 15.00 | 3700.00 |
| LM 001-04 | 1000.00 | - | - | - | - | 3700.00 |
| LM 001-05 | 4500.00 | 584.00 (4 mM) | - | 110.00 (1 mM) | 15.00 | 3700.00 |
| LM 001-06 | 1000.00 | 584.00 (4 mM) | - | - | 15.00 | 3700.00 |
| LM 001-07 | 4500.00 | 584.00 (4 mM) | - | - | 15.00 | 3700.00 |
| LM 001-08 | 4500.00 | - | - | 110.00 (1 mM) | 15.00 | 3700.00 |
| LM 001-09 | 4500.00 | 584.00 (4 mM) | 5958.00 (25 mM) | - | 15.00 | 3700.00 |
| LM 001-10 | 4500.00 | 584.00 (4 mM) | 5958.00 (25 mM) | - | - | 3700.00 |
| LM 001-11 | 1000.00 | 584.00 (4 mM) | - | 110.00 (1 mM) | 15.00 | 3700.00 |
| LM 001-12 | 1000.00 | 584.00 (4 mM) | 5958.00 (25 mM) | 110.00 (1 mM) | 15.00 | 3700.00 |
| LM 001-13 | 1000.00 | - | 5958.00 (25 mM) | 110.00 (1 mM) | 15.00 | 3700.00 |
| LM 001-14 | 1000.00 | - | - | 110.00 (1 mM) | 15.00 | 3700.00 |
| LM 001-15 | 1000.00 | - | - | 110.00 (1 mM) | - | 3700.00 |
Comparison of special ordered liquid DMEM
| Cat. No. | Basic Formulation | Special Modification |
|---|---|---|
| LM 001-50 | LM 001-07 | With 5958.00 mg/L (25 mM) HEPES |
| LM 001-51 | LM 001-05 | With 1500.00 mg/L sodium bicarbonate |
| LM 001-52 | LM 001-05 | With 5958.00 mg/L (25 mM) HEPES |
| LM 001-53 | LM 001-07 | Without sodium bicarbonate and phenol red |
| LM 001-54 | LM 001-05 | Without magnesium sulfate |
| LM 001-55 | LM 001-11 | With 4750.00 mg/L HEPES and 2000.00 mg/L sodium bicarbonate |
| LM 001-56 | LM 001-07 | Without D-glucose |
| LM 001-57 | LM 001-07 | Without L-isoleucine |
| LM 001-58 | LM 001-07 | Without i-inositol (myo-inositol) |
| LM 001-59 | LM 001-07 | Without phenol red |
| LM 001-60 | LM 001-07 | With 1500.00 mg/L sodium bicarbonate |
| LM 001-70 | LM 001-05 | Without phenol red |
| LM 001-17 | LM 001-11 | Without phenol red |
| LM 001-79 | LM 001-05 | Without D-glucose |
* In effort to cater to the individual needs of our clients, Welgene provides custom products (classified as a “special order”) with variations made to the general specifications listed on the catalogue. Variations can be made in regards to 1. elimination of certain materials 2. alteration of chemical portions 3. addition of new materials and 4. change in pH