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DMEM (LM001-11)

DMEM (LM001-11)


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Price
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500ML
₩16,800
1L
₩28,900
 
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Product Details

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Dulbecco's Modified Eagle's Medium (DMEM) (1X), Liquid (Low Glucose)
With 1000 mg/L D-glucose
With L-glutamine
With 110 mg/L sodium pyruvate

Catalog Number LM 001-11
Storage Temperature 2~8°C

Product Description
For animal cell culture, Eagle’s Medium have been developed and modified since the original formulation appeared in the literature. Dulbecco’s Modified Eagle’s Medium (DMEM) is a modification of Minimum Essential Medium (MEM) that contains a higher concentration of amino acids and vitamins, as well as additional supplementary components, such as D-glucose (low or high, 1000 mg/L or 4500 mg/L, respectively).

LM 001-11 contains the low D-glucose concentration of 1000 mg/L, 584 mg/L L-glutamine, and 110 mg/L sodium pyruvate. The selection of a nutrient medium is strongly influenced by (1) type of cell, (2) type of culture (monolayer, suspension, clonal), and (3) degree of chemical definition necessary. It is important to review the literature for recommendations concerning medium, supplementation and physiological parameters required for a specific cell line.

Storage/Stability
The liquid medium should be stored at 2~8°C in the dark. Deterioration of the liquid medium may be recognized by (1) precipitate or particulate matter throughout the solution, (2) cloudy appearance, (3) color change, and/or (4) pH change. The nature of supplements added may affect storage conditions and shelf life of the medium. Product label bears expiration date. 

Biological Performance Characteristics
The growth-promoting capacities of DMEM are tested in a medium containing 10% FBS using an appropriate cell line(s). Growth rates are examined through three subculture generations and compared with parallel cultures grown in standardized control medium. Cells are counted and growth is plotted as a logarithmic function of time in culture, and seeding efficiency, doubling time, and final cell densities are determined. During the testing period cultures are examined microscopically for a typical morphology and evidence of cytotoxicity.

Precautions
For In Vitro Use Only 

 

Product Name Description Cat. No. Recommended Storage Temp. Size
Dulbecco’s Modified Eagle’s Medium (DMEM) (1X),
liquid (high glucose)
With 4500 mg/L D-glucose
Without L-glutamine
LM 001-01 Store at 2-8°C 500ML, 1L
Dulbecco’s Modified Eagle’s Medium (DMEM) (1X),
liquid (low glucose)
With 1000 mg/L D-glucose
Without L-glutamine
LM 001-02 Store at 2-8°C 500ML
Dulbecco’s Modified Eagle’s Medium (DMEM) (1X),
liquid (high glucose)
With 4500 mg/L D-glucose
With 25 mM HEPES
Without L-glutamine
LM 001-03 Store at 2-8°C 500ML, 1L
Dulbecco’s Modified Eagle’s Medium (DMEM) (1X),
liquid (low glucose)
With 1000 mg/L D-glucose
Without L-glutamine
Without phenol red
LM 001-04 Store at 2-8°C 500ML
Dulbecco’s Modified Eagle’s Medium (DMEM) (1X),
liquid (high glucose)
With 4500 mg/L D-glucose
With L-glutamine
With 110 mg/L sodium pyruvate
LM 001-05 Store at 2-8°C 500ML, 1L
Dulbecco’s Modified Eagle’s Medium (DMEM) (1X),
liquid (low glucose)
With 1000 mg/L D-glucose
With L-glutamine
LM 001-06 Store at 2-8°C 500ML, 1L
Dulbecco’s Modified Eagle’s Medium (DMEM) (1X),
liquid (high glucose)
With 4500 mg/L D-glucose
With L-glutamine
LM 001-07 Store at 2-8°C 500ML, 1L
Dulbecco’s Modified Eagle’s Medium (DMEM) (1X),
liquid (high glucose)
With 4500 mg/L D-glucose
With 110 mg/L sodium pyruvate
Without L-glutamine
LM 001-08 Store at 2-8°C 500ML, 1L
Dulbecco’s Modified Eagle’s Medium (DMEM) (1X),
liquid (high glucose)
With 4500 mg/L D-glucose
With L-glutamine
With 25 mM HEPES
LM 001-09 Store at 2-8°C 500ML, 1L
Dulbecco’s Modified Eagle’s Medium (DMEM) (1X),
liquid (high glucose)
With 4500 mg/L D-glucose
With L-glutamine
With 25 mM HEPES
Without phenol red
LM 001-10 Store at 2-8°C 500ML
Dulbecco’s Modified Eagle’s Medium (DMEM) (1X),
liquid (low glucose)
With 1000 mg/L D-glucose
With L-glutamine
With 110 mg/L sodium pyruvate
LM 001-11 Store at 2-8°C 500ML, 1L
Dulbecco’s Modified Eagle’s Medium (DMEM) (1X),
liquid (low glucose)
With 1000 mg/L D-glucose
With L-glutamine
With 25 mM HEPES
With 110 mg/L sodium pyruvate
LM 001-12 Store at 2-8°C 500ML, 1L
Dulbecco’s Modified Eagle’s Medium (DMEM) (1X),
liquid (low glucose)
With 1000 mg/L D-glucose
With 25 mM HEPES
With 110 mg/L sodium pyruvate
Without L-glutamine
LM 001-13 Store at 2-8°C 500ML, 1L
Dulbecco’s Modified Eagle’s Medium (DMEM) (1X),
liquid (low glucose)
With 1000 mg/L D-glucose
With 110 mg/L sodium pyruvate
Without L-glutamine
LM 001-14 Store at 2-8°C 500ML, 1L
Dulbecco’s Modified Eagle’s Medium (DMEM) (1X),
liquid (low glucose)
With 1000 mg/L D-glucose
With 110 mg/L sodium pyruvate
Without L-glutamine
Without phenol red
LM 001-15 Store at 2-8°C 500ML, 1L

* In effort to cater to the individual needs of our clients, Welgene provides custom products (classified as a “special order”) with variations made to the general specifications listed on the catalogue. Variations can be made in regards to 1. elimination of certain materials 2. alteration of chemical portions 3. addition of new materials and 4. change in pH. 

 

Comparison of liquid DMEM

Cat. No. D-Glucose (mg/L) L-Glutamine (mg/L) HEPES (mg/L) Sodium Pyruvate (mg/L) Phenol Red (mg/L) Sodium Bicarbonate (mg/L)
LM 001-01 4500.00 - - - 15.00 3700.00
LM 001-02 1000.00 - - - 15.00 3700.00
LM 001-03 4500.00 - 5958.00 (25 mM) - 15.00 3700.00
LM 001-04 1000.00 - - - - 3700.00
LM 001-05 4500.00 584.00 (4 mM) - 110.00 (1 mM) 15.00 3700.00
LM 001-06 1000.00 584.00 (4 mM) - - 15.00 3700.00
LM 001-07 4500.00 584.00 (4 mM) - - 15.00 3700.00
LM 001-08 4500.00 - - 110.00 (1 mM) 15.00 3700.00
LM 001-09 4500.00 584.00 (4 mM) 5958.00 (25 mM) - 15.00 3700.00
LM 001-10 4500.00 584.00 (4 mM) 5958.00 (25 mM) - - 3700.00
LM 001-11 1000.00 584.00 (4 mM) - 110.00 (1 mM) 15.00 3700.00
LM 001-12 1000.00 584.00 (4 mM) 5958.00 (25 mM) 110.00 (1 mM) 15.00 3700.00
LM 001-13 1000.00 - 5958.00 (25 mM) 110.00 (1 mM) 15.00 3700.00
LM 001-14 1000.00 - - 110.00 (1 mM) 15.00 3700.00
LM 001-15 1000.00 - - 110.00 (1 mM) - 3700.00


Comparison of special ordered liquid DMEM

Cat. No. Basic Formulation Special Modification
LM 001-50 LM 001-07 With 5958.00 mg/L (25 mM) HEPES
LM 001-51 LM 001-05 With 1500.00 mg/L sodium bicarbonate
LM 001-52 LM 001-05 With 5958.00 mg/L (25 mM) HEPES
LM 001-53 LM 001-07 Without sodium bicarbonate and phenol red
LM 001-54 LM 001-05 Without magnesium sulfate
LM 001-55 LM 001-11 With 4750.00 mg/L HEPES and 2000.00 mg/L sodium bicarbonate
LM 001-56 LM 001-07 Without D-glucose
LM 001-57 LM 001-07 Without L-isoleucine
LM 001-58 LM 001-07 Without i-inositol (myo-inositol)
LM 001-59 LM 001-07 Without phenol red
LM 001-60 LM 001-07 With 1500.00 mg/L sodium bicarbonate
LM 001-70 LM 001-05 Without phenol red

* In effort to cater to the individual needs of our clients, Welgene provides custom products (classified as a “special order”) with variations made to the general specifications listed on the catalogue. Variations can be made in regards to 1. elimination of certain materials 2. alteration of chemical portions 3. addition of new materials and 4. change in pH