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F-10 Nutrient Mixtures
(HAM’S F-10) (1X), Liquid
Catalog Number LM 009-01
Storage Temperature 2~8°C
For the clonal growth of several clones of Chinese Hamster Ovary (CHO) cells, Ham, R.G. developed Ham’s nutrient mixtures were developed in 1960s. Instead of serum, it contains a supplement of nutrients, growth factors and hormones. Ham’s F-10 was originally used as serum-free, but now used with serum supplement for a variety of cell types. Ham’s F-10 has been shown to support the growth of human diploid cells, white blood cells for chromosomal analysis and primary explants of rat, rabbit and chicken tissues. HEPES buffer provides more effective buffering in the optimum pH range of 7.2~7.4.
LM 009-01 contains 146 mg/L L-glutamine but no HEPES. Add 20 ml of HEPES buffer (BB 001-01, 1 M) per 1 L of medium, if desired. The selection of a nutrient medium is strongly influenced by (1) type of cell, (2) type of culture (monolayer, suspension, clonal), and (3) degree of chemical definition necessary. It is important to review the literature for recommendations concerning medium, supplementation and physiological parameters required for a specific cell line.
The liquid medium should be stored at 2~8°C in the dark. Deterioration of the liquid medium may be recognized by (1) precipitate or particulate matter throughout the solution, (2) cloudy appearance, (3) color change, and/or (4) pH change. The nature of supplements added may affect storage conditions and shelf life of the medium. Product label bears expiration date.
Biological Performance Characteristics
The growth-promoting capacities of Ham’s F-10 are tested in a medium using an appropriate cell line(s). Growth rates are examined through three subculture generations and compared with parallel cultures grown in standardized control medium. Cells are counted and growth is plotted as a logarithmic function of time in culture, and seeding efficiency, doubling time, and the final cell density are determined. During the testing period cultures are examined microscopically for a typical morphology and evidence of cytotoxicity.
For In Vitro Use Only
* In effort to cater to the individual needs of our clients, Welgene provides custom products (classified as a “special order”) with variations made to the general specifications listed on the catalogue. Variations can be made in regards to 1. elimination of certain materials 2. alteration of chemical portions 3. addition of new materials and 4. change in pH.
Comparison of liquid Ham’s F-10
Comparison of special ordered liquid Ham’s F-10
* In effort to cater to the individual needs of our clients, Welgene provides custom products (classified as a “special order”) with variations made to the general specifications listed on the catalogue. Variations can be made in regards to 1. elimination of certain materials 2. alteration of chemical portions 3. addition of new materials and 4. change in pH