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McCoy’s 5A Medium, modified (1X) Liquid
Catalog Number LM 005-01 (without HEPES)
LM 005-02 (With HEPES)
Storage Temperature 2~8°C
McCoy and his coworkers developed McCoy’s 5A Medium for in vitro cultivation of Novikoff Hepatoma Cells. Basal Medium 5A was used and modified for these studies to support Walker Carcinosarcoma 256 Cells. In this medium, Hsu and Kellogg cultured successfully primary cultures derived from normal bone marrow, skin, gingiva, testes, mouse kidney, omentum, adrenal glands, lung, spleen, rat embryos, and other tissues.
LM 005-01 and LM 005-02 contain 219.2 mg/L Lglutamine. HEPES buffer is included in LM 005-02 at the concentration of 25 mM. The selection of a nutrient medium is strongly influenced by (1) type of cell, (2) type of culture (monolayer, suspension, or clonal) and (3) degree of chemical definition necessary. It is important to review the literature for recommendations concerning medium, supplementation and physiological parameters required for a specific cell line.
The liquid McCoy’s 5A medium should be stored at 2~8°C in the dark. Deterioration of the liquid medium may be recognized by (1) precipitate or particulate matter throughout the solution, (2) cloudy appearance, (3) color change, and/or (4) pH change. The nature of supplements added may affect storage conditions and shelf life of the medium. Product label bears expiration date.
Biological Performance Characteristics
The growth-promoting capacities of McCoy’s 5A medium are tested in a medium containing 10% FBS using an appropriate cell line(s). Growth rates are examined through three subculture generations and compared with parallel cultures grown in standardized control medium. Cells are counted and growth is plotted as a logarithmic function of time in culture, and seeding efficiency, doubling time, and the final cell density are determined. During the testing period cultures are examined microscopically for a typical morphology and evidence of cytotoxicity.
For In Vitro Use Only
* In effort to cater to the individual needs of our clients, Welgene provides custom products (classified as a “special order”) with variations made to the general specifications listed on the catalogue. Variations can be made in regards to 1. elimination of certain materials 2. alteration of chemical portions 3. addition of new materials and 4. change in pH.
Comparison of liquid McCoy’s 5A Media, modified
ordered liquid McCoy’s 5A Media, modified
* In effort to cater to the individual needs of our clients, Welgene provides custom products (classified as a “special order”) with variations made to the general specifications listed on the catalogue. Variations can be made in regards to 1. elimination of certain materials 2. alteration of chemical portions 3. addition of new