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Medium 199 (1X), Liquid

Medium 199 (1X), Liquid


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Price
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500ml
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1L
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Product Details

Medium 199 (1X), Liquid
With Earle’s salts
With sodium bicarbonate

Catalog Number LM 006-01 (with L-glutamine)
LM 006-02 (without L-glutamine)
Storage Temperature 2~8°C

Product Description
Medium 199 contains various combinations of vitamins, amino acids and other factors. It has broad species applicability, particularly for cultivation of non-transformed cells. Morgan and his coworkers reported their efforts to produce a totally defined nutritional source, instead of serum, for cell cultures. However, it was found that long-term cultivation of cells required addition of a serum supplement to the culture. It is widely used in virology, vaccine production and in vitro cultivation of primary explants of mouse pancreatic epithelial and rat lens tissues.

LM 006-01 is based on the Earle’s balanced salts, and contains 100 mg/L of L-glutamine, 2200 mg/L of sodium bicarbonate. LM 006-02 is based on the Earle’s balanced salts, and contains 2200 mg/L of sodium bicarbonate but no L-glutamine. Add 3.42 ml of L-glutamine (LS 002-01, 200 mM) per 1 L of medium, if desired. The selection of a nutrient medium is strongly influenced by (1) type of cell, (2) type of culture (monolayer, suspension, or clonal), and (3) degree of chemical definition necessary. It is important to review the literature for recommendations concerning medium, supplementation and physiological parameters required for a specific cell line.

Storage/Stability
The liquid medium should be stored at 2~8°C in the dark. Deterioration of the liquid medium may be recognized by (1) precipitate or particulate matter throughout the solution, (2) cloudy appearance, (3) color change, and/or (4) pH change. The nature of supplements added may affect storage conditions and shelf life of the medium. Product label bears expiration date. 

Biological Performance Characteristics
The growth-promoting capacities of Medium 199 are tested in a liquid medium using an appropriate cell line(s). Growth rates are examined through three subculture generations and compared with parallel cultures grown in standardized control medium. Cells are counted and growth is plotted as a logarithmic function of time in culture, and seeding efficiency, doubling time, and the final cell density are determined. During the testing period cultures are examined microscopically for a typical morphology and evidence of cytotoxicity.

 

Product Profile

LM 006-01

LM 006-02

Appearance

Red translucent solution

Red translucent solution

pH at RT

7.0 ~ 7.6

7.0 ~ 7.6

Osmolality

280 ~ 310 mOsm/kg H2O

280 ~ 310 mOsm/kg H2O

Endotoxin

£ 1.0 EU/ml

£ 1.0 EU/ml

Sterility

Sterilized by 0.2 mm filtration system. Sterility tests are performed in accordance with protocols described in USP.

 

 

 

Salts

L-glutamine

HEPES

Phenol red

LM 006-01

Earle’s

O

-

O

LM 006-02

Earle’s

-

-

O

LM 006-03

Earle’s

-

O

O

LM 006-04

Earle’s

O

O

O

LM 006-05

Earle’s

O

O

-

LM 006-06

Hanks’

O

-

O

LM 006-07

Hanks’

O

O

O

 

Precautions
For In Vitro Use Only

 

Components

LM 006-01

LM 006-02

CaCl2 (anhydrous)

200.00

200.00

Fe(NO3)3·9H2O

0.72

0.72

KCl

400.00

400.00

MgSO4 (anhydrous)

98.00

98.00

NaCl

6800.00

6800.00

NaHCO3

2200.00

2200.00

NaH2PO4·H2O

140.00

140.00

Adenine Sulfate

10.00

10.00

Adenosine-5-triphosphate

1.00

1.00

Adenosine-5-phosphate

0.20

0.20

Chloesterol

0.20

0.20

2-deoxy-D-ribose

0.50

0.50

D-Glucose

1000.00

1000.00

Glutathione (reduced)

0.05

0.05

Guanine·HCl

0.30

0.30

HEPES

-

-

Hypoxanthine·Na

0.40

0.40

Phenol Red

20.00

20.00

Ribose

0.50

0.50

Sodium Acetate

50.00

50.00

Thymine

0.30

0.30

Tween 80

20.00

20.00

Uracil

0.30

0.30

Xanthine·Na

0.340

0.340

L-Alanine

25.00

25.00

L-Arginine·HCl

70.00

70.00

L-Aspartic Acid

30.00

30.00

L-Cystein·HCl·H2O

0.10

0.10

L-Cysteine·2HCl

26.00

26.00

Glycine

50.00

50.00

L-Glutamic Acid

75.00

75.00

L-Glutamine

100.00

-

L-Histidine·HCl·H2O

22.00

22.00

L-Hydroxyproline

10.00

10.00

L-Isoleucine

40.00

40.00

L-Leucine

60.00

60.00

L-Lysine·HCl

70.00

70.00

L-Methionine

15.00

15.00

L-Phenylalanine

25.00

25.00

L-Proline

40.00

40.00

L-Serine

25.00

25.00

L-Threonine

30.00

30.00

L-Tryptophan

10.00

10.00

L-Tyrosine·2Na·2H2O

58.00

58.00

L-Valine

25.00

25.00

Ascorbic Acid

0.050

0.050

α –Tocopherol Phosphate (sodium salt)

0.01

0.01

Biotin

0.01

0.01

Calciferol

0.10

0.10

D-Ca Pantothenate

0.01

0.01

Choline Chloride

0.50

0.50

Folic Acid

0.01

0.01

i-Inositol

0.05

0.05

Menadione (sodium bisulfite)

0.01

0.01

Niacin

0.025

0.025

Niacinamide

0.025

0.025

Para-aminobenzoic Acid

0.05

0.05

Pyridoxal·HCl

0.025

0.025

Pyridoxine·HCl

0.025

0.025

Riboflavin

0.01

0.01

Thiamine·HCl

0.01

0.01

Vitamin A (acetate)

0.14

0.14

 

 

References

Morgan, J.F, Morton, H.J. and Parker, R.C. (1950). The Nutrition of Animal Cells in Tissue Culture. I. Initial Studies on a Synthetic Medium. Proc. Soc. Exp. Biol. Med. 73, 1-8.
Morgan, J.F., Campbell, E. and Morton, H.J. (1955). The Nutrition of Animal Tissues Cultivated In Vitro. I. A Survey of Natural Materials as Supplements to Synthetic Medium. J.N.C.I. 16:2, 557-567.
Morton, H.J. (1970). A Survey of Commercially Available Tissue Culture Media. In Vitro. 6, 89-108.
Rutzky, L.P. and Pumper, R.W., (1974). Supplement to a Survey of Commercially Available Tissue Culture Media (1970). In Vitro. 9, 468-469.