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MEM - 2X (LM203-50)

MEM - 2X (LM203-50)

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Minimum Essential Medium Eagle (MEM) (2X), Liquid
With L-glutamine
With sodium bicarbonate

Catalog Number LM 203-50 (With Earle's salts
                                  Equal to LM007-01 at 1X)

Catalog Number LM 203-51 (With Hanks' salts
                                 Equal to LM007-05 at 1X)

Storage Temperature 2~8°C

Product Description
Minimum Essential Medium (MEM) was developed by Harry Eagle and has been used for wide variety of cells grown in monolayer. Basal Medium Eagle (BME), previously developed, could not meet the specific nutritional requirements for cultivating normal mammalian fibroblasts and certain subtypes of HeLa cells. MEM includes high concentration of amino acids so that it can more closely approximate the protein composition of cultured mammalian cells. The formulation of MEM containing either Hanks’ or Earle’s salts can be supplemented by optional non-essential amino acids for the variable types of cells. The formulation can be further modified by optional elimination of calcium, necessary for attachment, to permit growth of cells in suspension culture.

LM 203-50 is based on the Earle’s balanced salts, and contains 584 mg/L of L-glutamine, 4400 mg/L of sodium bicarbonate. LM 203-51 is based on the Hanks' balanced salts, and contains 584 mg/L of L-glutamine, 700 mg/L of sodium bicarbonate. The selection of a nutrient medium is strongly influenced by (1) type of cell, (2) type of culture (monolayer, suspension, or clonal), and (3) degree of chemical definition necessary. It is important to review the literature for recommendations concerning medium, supplementation and physiological parameters required for a specific cell line.

The liquid medium should be stored at 2~8°C in the dark. Deterioration of the liquid medium may be recognized by (1) precipitate or particulate matter throughout the solution, (2) cloudy appearance, (3) color change, and/or (4) pH change. The nature of supplements added may affect storage conditions and shelf life of the medium. Product label bears expiration date.

Biological Performance Characteristics
The growth-promoting capacities of MEM are tested in a medium containing 5~10% FBS using an appropriate cell line(s). Growth rates are examined through three subculture generations and compared with parallel cultures grown in standardized control medium. Cells are counted and growth is plotted as a logarithmic function of time in culture, and seeding efficiency, doubling time, and the final cell density are determined. During the testing period cultures are examined microscopically for a typical morphology and evidence of cytotoxicity.

Preparation Procedure (1 L of 1X medium)

  1. 1. Mix 500 ml of 2X Minimum Essential Media Eagle and 400 ml of sterile cell/tissue culture grade water (LS 016-01) in an appropriate vessel.

       - If the desired pH is different from the pH in table below, adjust pH using sterile 1 N HCl (LS 003-02) or 1 N NaOH(LS 012-02).

  1. Fill up to 1 L by adding sterile cell/tissue culture grade water.

For In Vitro Use Only