Minimum Essential Medium Eagle (LM007-14)
냉장, 냉동 제품은 금요일에는 발송이 불가능하며, 차주 월요일에 발송됩니다.
재고 소진 시 배송이 지연 될 수 있습니다.(제품마다 생산소요기간 상이)
| 제품 설명서 (PDF 파일 다운로드) | MSDS (PDF 파일 다운로드) | |
Minimum Essential Medium Eagle (MEM), Liquid
With Earle’s salts
Catalog Number
LM 007-12 (without L-glutamine and phenol red)
LM 007-13 (with 10 mM HEPES and L-glutamine)
LM 007-14 (with 1500 mg/L sodium bicarbonate and without L-glutamine)
Storage Temperature 2~8°C
Product Description
Minimum Essential Medium (MEM) was developed by Harry Eagle and has been used for wide variety of cells grown in monolayer. Basal Medium Eagle (BME), previously developed, could not meet the specific nutritional requirements for cultivating normal mammalian fibroblasts and certain subtypes of HeLa cells. MEM includes high concentration of amino acids so that it can more closely approximate the protein composition of cultured mammalian cells. The formulation of MEM containing either Hanks’ or Earle’s salts can be supplemented by optional non-essential amino acids for the variable types of cells. The formulation can be further modified by optional elimination of calcium, necessary for attachment, to permit growth of cells in suspension culture.
LM 007-12 is based on the Earle’s balanced salts, and does not contain L-glutamine and phenol red. LM 007-13 is based on the Earle’s balanced salts, and contains 10 mM HEPES and 292 mg/L L-glutamine. LM 007-14 is based on the Earle’s balanced salts, and contains 1500 mg/L sodium bicarbonate, but no L-glutamine. The selection of a nutrient medium is strongly influenced by (1) type of cell, (2) type of culture (monolayer, suspension, or clonal), and (3) degree of chemical definition necessary. It is important to review the literature for recommendations concerning medium, supplementation and physiological parameters required for a specific cell line.
Storage/Stability
The liquid medium should be stored at 2~8°C in the dark. Deterioration of the liquid medium may be recognized by (1) precipitate or particulate matter throughout the solution, (2) cloudy appearance, (3) color change, and/or (4) pH change. The nature of supplements added may affect storage conditions and shelf life of the medium. Product label bears expiration date.
Biological Performance Characteristics
The growth-promoting capacities of MEM are tested in a medium containing 5~10% FBS using an appropriate cell line(s). Growth rates are examined through three subculture generations and compared with parallel cultures grown in standardized control medium. Cells are counted and growth is plotted as a logarithmic function of time in culture, and seeding efficiency, doubling time, and the final cell density are determined. During the testing period cultures are examined microscopically for a typical morphology and evidence of cytotoxicity.
Precautions
For In Vitro Use Only
| Product Name | Description | Cat. No. | Recommended Storage Temp. | Size |
|---|---|---|---|---|
| Minimum Essential Medium Eagle (MEM) (1X), liquid | With Earle’s salts With L-glutamine |
LM 007-01 | Store at 2-8°C | 500ML, 1L |
| Minimum Essential Medium Eagle (MEM), Joklik Modification (1X), liquid | Modified for suspension cultures With L-glutamine With 10X phosphate Without calcium chloride |
LM 007-02 | Store at 2-8°C | 500ML, 1L |
| Minimum Essential Medium Eagle, Spinner Modification (S-MEM) (1X), liquid | Modified for suspension cultures With Earle’s salts With L-glutamine |
LM 007-03 | Store at 2-8°C | 500ML, 1L |
| Minimum Essential Medium Eagle (MEM) (1X), liquid | With Hanks’ salts With L-glutamine |
LM 007-05 | Store at 2-8°C | 500ML, 1L |
| Minimum Essential Medium Eagle (MEM) (1X), liquid | With Hanks’ salts With L-glutamine With 25 mM HEPES |
LM 007-06 | Store at 2-8°C | 500ML |
| Minimum Essential Medium Eagle (MEM) (1X), liquid | With Earle’s salts With non-essential amino acids With L-glutamine With L-glutamine |
LM 007-07 | Store at 2-8°C | 500ML, 1L |
| Minimum Essential Medium Eagle (MEM) (1X), liquid | With Earle’s salts With non-essential amino acids Without L-glutamine |
LM 007-08 | Store at 2-8°C | 500ML |
| Minimum Essential Medium Eagle (MEM) (1X), liquid | With Earle’s salts With 25 mM HEPES With L-glutamine |
LM 007-09 | Store at 2-8°C | 500ML, 1L |
| Minimum Essential Medium Eagle (MEM) (1X), liquid | With Earle’s salts With 25 mM HEPES Without L-glutamine |
LM 007-10 | Store at 2-8°C | 500ML, 1L |
| Minimum Essential Medium Eagle (MEM) (1X), liquid | With Earle’s salts Without L-glutamine |
LM 007-11 | Store at 2-8°C | 500ML, 1L |
| Minimum Essential Medium Eagle (MEM) (1X), liquid | With Earle’s salts Without L-glutamine Without phenol red |
LM 007-12 | Store at 2-8°C | 500ML |
| Minimum Essential Medium Eagle (MEM) (1X), liquid | With Earle’s salts With 10 mM HEPES With L-glutamine |
LM 007-13 | Store at 2-8°C | 500ML, 1L |
| Minimum Essential Medium Eagle (MEM) (1X), liquid | With Earle’s salts With 1500 mg/L sodium bicarbonate Without L-glutamine |
LM 007-14 | Store at 2-8°C | 500ML, 1L |
* In effort to cater to the individual needs of our clients, Welgene provides custom products (classified as a “special order”) with variations made to the general specifications listed on the catalogue. Variations can be made in regards to 1. elimination of certain materials 2. alteration of chemical portions 3. addition of new materials and 4. change in pH.
Comparison of liquid MEM
| Cat. No. | Balanced Salt Solution | L-Glutamine (mg/L) |
HEPES (mg/L) |
CaCl2 (anhyd.) (mg/L) |
Phenol Red (mg/L) |
L-Methionine (mg/L) |
NEAAs (mg/L) |
Sodium Bicarbonate (mg/L) |
|---|---|---|---|---|---|---|---|---|
| LM 007-01 | Earle's Salts | 292.00 (2 mM) | - | 200.00 | 10.00 | 15.00 | - | 2200.00 |
| LM 007-02 | Joklik modification | 292.00 (2 mM) | - | - | 10.00 | 15.00 | - | 2200.00 |
| LM 007-03 | Spinner modification | 292.00 (2 mM) | - | - | 10.00 | 15.00 | - | 2200.00 |
| LM 007-05 | Hanks' Salts | 292.00 (2 mM) | - | 140.00 | 10.00 | 15.00 | - | 350.00 |
| LM 007-06 | Hanks' Salts | 292.00 (2 mM) | 5958.00 (25 mM) | 140.00 | 10.00 | 15.00 | - | 350.00 |
| LM 007-07 | Earle's Salts | 292.00 (2 mM) | - | 200.00 | 10.00 | 15.00 | 0.1 mM | 2200.00 |
| LM 007-08 | Earle's Salts | - | - | 200.00 | 10.00 | 15.00 | 0.1 mM | 2200.00 |
| LM 007-09 | Earle's Salts | 292.00 (2 mM) | 5958.00 (25 mM) | 200.00 | 10.00 | 15.00 | - | 2200.00 |
| LM 007-10 | Earle's Salts | - | 5958.00 (25 mM) | 200.00 | 10.00 | 15.00 | - | 2200.00 |
| LM 007-11 | Earle's Salts | - | - | 200.00 | 10.00 | 15.00 | - | 2200.00 |
| LM 007-12 | Earle's Salts | - | - | 200.00 | - | 15.00 | - | 2200.00 |
| LM 007-13 | Earle's Salts | 292.00 (2 mM) | 2383.20 (10 mM) | 200.00 | 10.00 | 15.00 | - | 2200.00 |
| LM 007-14 | Earle's Salts | - | - | 200.00 | 10.00 | 15.00 | - | 1500.00 |
* NEAAs, Non-essential amino acids (L-Ala, L-Asn, L-Asp, L-Glu, Gly, L-Pro, and L-Ser)
Comparison of special ordered liquid MEM
| Cat. No. | Basic Formulation | Special Modification |
|---|---|---|
| LM 007-04 | LM 007-01 | Without L-Methionine |
| LM 007-50 | * | Sterilized by autoclaving Without sodium bicarbonate |
| LM 007-51 | LM 007-03 | With non-essential amino acids |
| LM 007-54 | LM 007-07 | With Sodium pyruvate 1mM, with Sodium bicarbonate 1,500 mg/L |
| LM 007-60 | LM 007-07 | Without D-glucose |
| LM 007-62 | LM 007-01 | With D-Valine and without L-Valine |
| LM 007-61 | LM 007-11 | Without D-glucose |
| LM 007-67 | LM 007-07 | Without amino acids |
* In effort to cater to the individual needs of our clients, Welgene provides custom products (classified as a “special order”) with variations made to the general specifications listed on the catalogue. Variations can be made in regards to 1. elimination of certain materials 2. alteration of chemical portions 3. addition of new materials and 4. change in pH.