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Cell culture tested
For gram-positive and gram-negative bacteria
LS 202-01 (50X)
With 5,000 units/mL of penicillin (base)
5,000 μg/mL streptomycin (base)
LS 202-02 (100X)
With 10,000 units/mL of penicillin (base)
10,000 μg/mL streptomycin (base)
Storage Temperature -5~-20°C
Microbial contamination results in the lethal errors in cell and tissue culture. Animal cell divides into two daughter cells in several hours to days, however microbes such as bacteria, fungi, or yeasts are doubled in decades of minute to hours. Hence, overgrowing of these microbes inhibits the growth, proliferation, and function of animal cells. Microbial components or products can result the toxic effect to animal cells. Generally, in culture medium, antibiotics are added for inhibition of microbial growth. Though there are many types of antibiotics used in cell culture media, penicillin G and streptomycin are widely used.
LS 202-01 and LS 202-02 may be used as the antibiotic solution for preventing the contamination of gram- positive and gram-negative bacteria. The generally used concentrations of these antibiotics are 100,000 units/L for penicillin and 100 mg/L for streptomicin. Each concentration of antibiotics can be obtained by 1/50 dilution (LS 202-01) or by 1/100 dilution (LS 202-02) in the medium to be used.
* G (+), Gram-positive bacteria
* G (+, -), Gram-positive and Gram-negative bacteria
The liquid antibiotic solution should be stored at -5~-20°C in the dark and is stable for 3 days during cultivation of cells at 37°C. After 3 days, medium should be refreshed to a new medium containing a new antibiotic solution. Deterioration of the liquid may be recognized by (1) precipitate or particulate matter throughout the solution, (2) cloudy appearance, (3) color change, and/or (4) pH change. The nature of supplements added may affect storage conditions and shelf life of the medium. Product label bears expiration date.