Prestained Protein Size Marker

Catalog Number PM 100-01

Storage Temperature -20°C

Product Description

Prestained Protein Size Marker is a prestained marker for a

SDS-PAGE analysis. Well defined 10 recombinant proteins

are included and supplied in a ready-to-use formula (no need

to boil before use). It is suitable for sizing proteins between 9

and 170 kDa by SDS-PAGE.

Chromophores are covalently linked to proteins in prestained

size marker, and 10 prestained proteins are visible during

electrophoresis or electrophoretic transfer from the gel to a

membrane. The 75 kDa protein labeled with orange color

plays a ruler function for easy identification of banding.

Because coupling of chromophores to the proteins in

pre-stained marker affects their apparent molecular weights in

SDS-PAGE relative to unstained proteins, unstained protein

markers should be used for accuracy >95%.

Storage/Stability

Prestained Protein Size Marker should be stored immediately

upon receipt at -20

oC. If stored in the recommended

temperature, this product will be stable until the expiration date

printed out on the label.

Recommended loading volume

- 5 l/lane for a mini-gel (10 X 8 cm2

, 0.75 or 1 mm thick)

- 10-15 l/lane for a large-gel (30 X 20 cm2

, 1 or 1.5 mm thick)

- 2-5 l/lane for a western transfer onto PVDF or NC membranes

Cautions

- Repeated freezing & thawing, long-term storage and re-use

of pipette tip may degrade proteins rapidly.

- The marker is provided as a ready to load format, meaning

that there is no need to boil before use. But precipitation of

SDS may occur by long-term storage at below 4oC, and you

can load marker just after dissolving the precipitant by

raising to 50-80oC for a few minutes.

- The slight size difference can be seen in the course of

manufacturing. Please confirm the batch numbers

Fig A. Electrophoresis pattern in 15% SDS-PAGE gel Fig B. Molecular weight and color of individual prestained protein