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RPMI 1640 Amino Acid Solution (50X)
Cell culture tested
Catalog Number LS 009-01
Storage Temperature 2~8°C
Twenty amino acids are required for cell growth and proliferation. These are divided into two groups: a) Essential amino acids: Those that are not manufactured by animal cells and need to be supplemented in culture media. b) Non essential amino acids: These can be synthesized by animal cells, and need not to be supplemented in culture media. The concentration of amino acids usually limits the maximum cell concentration attainable, and the balance may influence cell survival and growth rate.
LS 009-01 contains RPMI 1640 essential amino acids in cell/tissue culture grade water (LS 016-01). The selection of a nutrient medium is strongly influenced by (1) type of cell, (2) type of culture (monolayer, suspension, or clonal) and (3) degree of chemical definition necessary. It is important to review the literature for recommendations concerning medium, supplementation and physiological parameters required for a specific cell line.
The RPMI 1640 amino acid solution should be stored at 2~8°C. Deterioration of the liquid may be recognized by (1) precipitate or particulate matter throughout the solution, (2) cloudy appearance, (3) color change, and/or (4) pH change. The nature of supplements added may affect storage conditions and shelf life of the medium. Product label bears expiration date.
Biological Performance Characteristics
The growth-maintaining capacity of RPMI 1640 amino acid solution is tested in RPMI 1640 containing 5% FBS using an appropriate cell line(s). Growth rates are examined through three subculture generations and compared with parallel cultures grown in standardized control medium. Cells are counted and growth is plotted as a logarithmic function of time in culture, and seeding efficiency, doubling time, and the final cell density are determined. During the testing period, cultures are examined microscopically for a typical morphology and evidence of cytotoxicity.
For In Vitro Use Only