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RPMI 1640 Vitamin Solution (100X)
Cell culture tested
Catalog Number LS 010-01
Storage Temperature -5~-20°C
Adding supplements of vitamins to media both stimulates growth and prolongs the viability of cells in culture. This concentrated supplement adds nutrients and reduces the biosynthetic burden on cell in vitro.
LS 010-01 contains D-biotin, choline chloride, folic acid, i-inositol, niacinamide, p-amino benzoic acid, D-pantothenate, pyridoxine, riboflavin, thiamine, and vitamin B12 in cell/tissue culture grade water (LS 016-01) with 0.85% NaCl.
The RPMI 1640 vitamin solution should be stored at -5~-20°C in the dark. Deterioration of the liquid may be recognized by (1) precipitate or particulate matter throughout the solution, (2) cloudy appearance, (3) color change, and/or (4) pH change. The nature of supplements added may affect storage conditions and shelf life of the medium. Product label bears expiration date.
Biological Performance Characteristics
The growth-maintaining capacity of RPMI 1640 vitamin solution is tested in RPMI 1640 containing 5% FBS using an appropriate cell line(s). Growth rates are examined through three subculture generations and compared with parallel cultures grown in standardized control medium. Cells are counted and growth is plotted as a logarithmic function of time in culture, and seeding efficiency, doubling time, and the final cell density are determined. During the testing period, cultures are examined microscopically for a typical morphology and evidence of cytotoxicity.
For In Vitro Use Only