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SOC Medium, Liquid
Catalog Number MM 006-01
Storage Temperature 2~8°C
SOC medium is widely used to support the transformation of E. coli with a foreign DNA such as plasmid DNA. Foreign DNA is inserted by CaCl2 and heat-shock, after which four time the amount of SOC liquid medium is added for cultivation. Spread the medium onto a SOB agar plate after 45 minutes.
SOC medium should be stored at 2~8°C. Deterioration of SOC medium may be recognized by (1) precipitate or particulate matter throughout the solution, (2) cloudy appearance, (3) color change, and/or (4) pH change. Product label bears expiration date.
Biological Performance Characteristics
The components of SOC medium should not be autoclaved together because the high temperature can cause the glucose to react with tryptic peptides (see Maillard reaction), compromising the quality. SOB and magnesium and glucose additive solutions can be autoclaved separately and mixed afterwards to final concentrations. Complete SOC can be filter sterilized through a 0.22 µm filter. SOC Medium is tested to ensure conformance with the most current approved product specification. This currently consists of tests for pH, sterility, and transformation efficiency using TOP10 E. coli.
For In Vitro Use Only