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TC-100 Insect Medium (1X), Liquid
With sodium bicarbonate
Insect cell culture tested
Catalog Number LM 505-01
Storage Temperature 2~8°C
Because of the rapid doubling time and the ability of over expression of proteins, many insect cell culture media were formulated to mimic the main physico- chemical properties of the body fluid of insects. Insect cells with baculoviruses have been employed to study a variety of biological processes including genetics, endocrinology, physiology and cell biology as well as recombinant protein expression. TC-100 Insect Medium has been used originally for the growth and maintenance of the cell lines derived from Spodoptera frugiperda. Other cell lines from lepidopterans are used for the production of Autographa californica Nuclear Polyhedrosis Virus in this medium.
LM 505-01 contains 600 mg/L L-glutamine and 350 mg/L sodium bicarbonate. The selection of a nutrient medium is strongly influenced by (1) type of cell, (2) type of culture (monolayer, suspension, or clonal), and (3) degree of chemical definition necessary. It is important to review the literature for recommendations concerning medium, supplementation and physiological parameters required for a specific cell line.
The liquid TC-100 medium should be stored at 2~8°C in the dark. Deterioration of the liquid medium may be recognized by (1) precipitate or particulate matter throughout the solution, (2) cloudy appearance, (3) color change, and/or (4) pH change. The nature of supplements added may affect storage conditions and shelf life of the medium. Product label bears expiration date.
Biological Performance Characteristics
The growth-promoting capacities of the liquid TC-100 Insect Medium are tested in a medium containing 5~20% heat-inactivated FBS using an appropriate insect cell line(s). Growth rates are examined through three subculture generations and compared with parallel cultures grown in standardized control medium. Cells are counted and growth is plotted as a logarithmic function of time in culture, and seeding efficiency, doubling time, and the final cell density are determined. During the testing period cultures are examined microscopically for a typical morphology and evidence of cytotoxicity.
For In Vitro Use Only