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TNM-FH Insect Medium, Liquid
With sodium bicarbonate
Insect cell culture tested
Catalog Number LM 506-01 (1X)
LM 506-02 (1.5X)
Storage Temperature 2~8°C
Grace’s Medium was developed for culturing the cells derived from the Australian moth, Antherea eucalypti. Grace’s Medium is a modification of Wyatt’s medium to more closely resemble Antherea hemolymph. Many modifications of Grace’s medium were developed in the late 1960’s and 1970’s. TNM-FH Insect Medium was one of these derivatives, developed for the in vitro cultivation of cells derived from the cabbage looper, Trichoplusia ni. by W.F. Hink in 1970. The medium when properly supplemented has been found to support the growth of cells derived from a variety of lepidopteran species.
LM 506-01 contains 600 mg/L L-glutamine and 350 mg/L sodium bicarbonate. LM 506-02 contains 900 mg/L L-glutamine, 525 mg/L sodium bicarbonate and 1.5-fold contents of LM 506-01 components. The selection of a nutrient medium is strongly influenced by (1) type of cell, (2) type of culture (monolayer, suspension, or clonal), and (3) degree of chemical definition necessary. It is important to review the literature for recommendations concerning medium, supplementation and physiological parameters required for a specific cell line.
The liquid medium should be stored at 2~8°C in the dark. Deterioration of the liquid medium may be recognized by (1) precipitate or particulate matter throughout the solution, (2) cloudy appearance, (3) color change, and/or (4) pH change. The nature of supplements added may affect storage conditions and shelf life of the medium. Product label bears expiration date.
Biological Performance Characteristics
The growth-promoting capacities of TNM-FH Insect Media are tested in a medium containing 10% FBS using an appropriate insect cell line(s). Growth rates are examined through three subculture generations and compared with parallel cultures grown in standardized control medium. Cells are counted and growth is plotted as a logarithmic function of time in culture, and seeding efficiencies, doubling time, and final cell densities are determined. During the testing period cultures are examined microscopically for a typical morphology and evidence of cytotoxicity.
For In Vitro Use Only