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5X M9 Minimal Salt (MM003-01)

5X M9 Minimal Salt (MM003-01)

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5X M9 Minimal Salt Solution
Catalog Number MM 003-01
Storage Temperature 15~30°C

Product Description
5X M9 Minimal Salts solution is used in the preparation of M9 minimal media (a liquid growth media for bacterial culture). The composition of the M9 Minimal Salts include buffering agents, a nitrogen source and necessary ions critical to the completion of M9 minimal media.

5X M9 Minimal Salts solution should be stored at 15~30°C. Deterioration of 5X M9 Minimal Salts solution may be recognized by (1) precipitate or particulate matter throughout the solution, (2) cloudy appearance, (3) color change, and/or (4) pH change. The nature of supplements added may affect storage conditions and shelf life of 5X M9 Minimal Salts solution. Product label bears expiration date.

Biological Performance Characteristics
Use the following instructions for preparing complete M9 minimal medium as a starting point. Additional supplementation may be required depending on the nutritional needs of the specific microbe to be cultured. Inoculate and incubate cultures on a rotary shaker at 33°C to 37°C for 18~48 hours.

For In Vitro Use Only 


Components MM 003-01 (g/L)
Na2HPO4·7H2O 64.0
KH2PO4 15.0
NaCl 2.5
NH4Cl 5.0


Product Name Description Cat. No. Recommended Storage Temp. Size
5X M9 Minimal Salt Solution Components
  64.0 g/L Na2HPO4•7H2O
  15.0 g/L KH2PO4
  2.5 g/L NaCl
  5.0 g/L NH4Cl
pH 7.0
Autoclaved (121°C, 15 lb/sq. in., 20 min)
Bacterial culture tested
Bacteriophage culture tested
MM 003-01 Store at 15-30°C 500ML