BCS Heat Inactivated (S103-01)
냉장, 냉동 제품은 금요일에는 발송이 불가능하며, 차주 월요일에 발송됩니다.
재고 소진 시 배송이 지연 될 수 있습니다.(제품마다 생산소요기간 상이)
제품 설명서 (PDF 파일 다운로드) | MSDS (PDF 파일 다운로드) |
Proper storage and thawing method of BCS
- After BCS thawing, why does clots of fleece shape appear? And how affect it to cell culture?
During BCS thawing, the most important thing is to shake slowly every 5~10 minutes until thawed all, minimizing bubbles. If the process does not work correctly, concentration gradient with insoluble crystals of fleece shape(cryoprecipitates) can be generated result from imbalance of salt and protein.
The cryoprecipitates is little effect on cell culture. In particular, thawed BCS should not be refrigerated and if you don’t follow the precautions, the clots can be caused a lot of further. Because of this, aliquots of thawing must be frozen keep. The cryoprecipitates is not a defect or fault of product.
- What is proper storage and use method of BCS?
1) Proper BCS thawing process(for minimizing deposits)
While BCS thawing, shake it slowly every 5 ~ 10 minutes minimizing bubbles for breaking concentration gradient.
★ Fill water in water bath almost to top of BCS(except cap) and raise temperature of water both by 37℃..
★ Put frozen BCS in water bath and thaw it by shaking slowly ever 5~10 minutes until breaking concentration gradient.
★ After thawing all, take out and spray 70% alcohol on the surface of bottle.
★ Divide BCS into 20~100ml in clean bench.
★ Freeze divided BCS as soon as possible. And keep BSC in refrigerator if it is used in a day or two. But keep in mind BCS kept in refrigerator can generate more clots than one in freezer.
★ If Heat-Inactivation process is needed, proceed as follows (in case of buying products that Heat-Inactivation completed, this process is unnecessary)
- Put thawed BCS in 56℃ water bath and proceed Heat-Inactivation for 30 minutes.
- Shake BCS slowly every 5~10 minutes minimizing bubbles and not making concentration gradient like thawing process. If this Heat-inactivation proceeded irregularly, lots of clots can be generated than thawing process.
2) Must storage BCS in a freezer without automatic frost-free function. (Avoid storage in a home refrigerator/freezer, which has frost-free function.)
Must Storage in a freezer without automatic frost-free function for preventing deterioration of BCS from temperature change. A freezer with automatic frost-free function change the temperature automatically for preventing frost, so it can cause reduce the quality of BCS by freezing and thawing the surface of product repeatedly. And give attention that product bottle can be broken in temperature.
3) Minimize freezing-thawing process
Temperature change from freezing-thawing process destroys protein, growth factor, hormone and other nutrient. Thus, it is necessary to minimize freezing-thawing process. After first thawing, divide BCS into amount used to once or twice(20~100ml) and frozen. This can reduce iterative freezing-thawing process and thawing hours. At this time attention not to exceed maximum volume 40ml in 50ml conical tube and 90ml in 100ml glass bottle considering of BCS expands as it freezes.