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Contains 10 mg/mL chloramphenicol in absolute ethanol
DNase, RNase and protease – none detected
Catalog Number ML 003-02
Storage Temperature -5~-20°C
Chloramphenicol is a synthetic antibiotic, which was first isolated from strains of Streptomyces venezuelae. It has a broad spectrum of activity against Gram-positive and Gram-negative bacteria. Chloramphenicol inhibits protein synthesis (elongation) by inhibiting translation on the 50S ribosomal subunit at the peptidyltransferase step. This antibiotic is often used in molecular biology applications for bacterial selection (10~20 μg/mL). The mode of resistance is inactivation of chloramphenicol (acetylation) by chloramphenicol acetyltransferase (cate gene).
ML 003-02 contains 10 mg/mL chloramphenicol in absolute ethanol. Working concentration is 10~20 μg/mL.
Chloramphenicol solution should be stored at -5~-20°C. Deterioration of the liquid may be recognized by (1) precipitate or particulate matter throughout the solution, (2) cloudy appearance, (3) color change, and/or (4) pH change. Product label bears expiration date.
For In Vitro Use Only