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Gentamicin (10 mg/ml), (LS201-01)

Gentamicin (10 mg/ml), (LS201-01)

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Gentamicin Solution (10 mg/ml)
Contains 10 mg/ml gentamicin sulfate
in cell/tissue culture grade water
For gram-positive and gram-negative bacteria

Catalog Number LS 201-01
Storage Temperature -5~-20°C

Product Description
Microbial contamination results in the lethal errors in cell and tissue culture. Animal cell divides into two daughter cells in several hours to days, however microbes such as bacteria, fungi, or yeasts are doubled in decades of minute to hours. Hence, overgrowing of these microbes inhibits the growth, proliferation, and function of animal cells. Microbial components or products can result the toxic effect to animal cells. Generally, in culture medium, antibiotics are added for inhibition of microbial growth. There are many types of antibiotics used in cell culture media, and gentamicin is one of the antibiotics widely used for preventing growth of gram-positive and gram-negative bacteria. Gentamicin interferes with bacterial protein synthesis by binding to 30S subunit of ribosomes.

LS 201-01 contains 10 mg/mL gentamicin sulfate in cell/tissue culture grade water (LS 016-01). The generally used concentration of gentamicin is 50 mg/L, which can be obtained by adding 5 mL of gentami

cin solution to 1 L of medium.

Antibiotics used in cell/tissue culture

Antibiotics Target Microbes* Actions
Gentamicin G (+, -) Interferes with bacterial protein synthesis by binding to 30S subunit of ribosomes
Penicillin G (+) Interferes with the final stage of synthesis of the bacterial cell wall
Streptomycin G (+, -) Binds to 30S subunit to cause misreading
Amphotericin B Y, M Interferes with the permeability of cell membrane of sensitive fungi by binding sterols

* G (+), Gram-positive bacteria
* G (+, -), Gram-positive and Gram-negative bacteria
* Y, Yeasts
* M, Molds

Gentamicin solution should be stored at -5~-20°C in the dark and can be stable for 5 days during cultivation of cells at 37°C. After 5 days, medium should be refreshed to a new medium containing a fresh gentamicin solution. Deterioration of the liquid may be recognized by (1) precipitate or particulate matter throughout the solution, (2) cloudy appearance, (3) color change, and/or (4) pH change. The nature of supplements added may affect storage conditions and shelf life of the medium. Product label bears expiration date.